In situ formation of biphasic calcium phosphates and their biological performance in vivo

• Published in: Ceramics international 2012-04, Vol.38 (3), p.1965-1974
• Main Authors: Kim, Tae-Wan, Park, Young Min, Kim, Dong-Hyun, Jin, Hyeong-Ho, Shin, Keun-Koo, Jung, Jin Sup, Park, Hong-Chae, Yoon, Seog-Young
• Format: Article
• Language: English
• Subjects: Biocompatibility; Biological; Biomedical materials; Biphasic calcium phosphates; Bone regeneration; Bones; Co-precipitation; Coprecipitation; Granular materials; Granulates; Granulation; Surgical implants
• ISSN: 0272-8842; 1873-3956
• DOI: 10.1016/j.ceramint.2011.10.029

The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). After annealing at 900°C for 24h, hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) were obtained as a single phase at 1.67 and 1.5 Ca/P ratios, respectively. Between these two extremes, a whole range of BCP preparations could be synthesized by using this technique with an accurate control of starting reactants. The biological performance of BCP granulates with a specific content of 62% HAp and 38% β-TCP was investigated. After immersion in Hanks’ balanced salt solution (HBSS) for 1 week, a precipitation started to be formed with individual small granules on the specimen surface. An MTT assay indicated that BCP granulates have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that BCP granulate provides a strong positive effect on bone formation in vivo in mice.