Flexible Microfabricated Film Sensors for the in Situ Quantum Dot-Based Voltammetric Detection of DNA Hybridization in Microwells

A new flexible miniaturized integrated device was microfabricated for the in situ ultrasensitive voltammetric determination of DNA mutation in a microwell format, using quantum dots (QDs) labels. The integrated device consisted of thin Bi, Ag, and Pt films (serving as the working, reference, and cou...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-01, Vol.87 (2), p.853-857
Main Authors: Kokkinos, Christos, Economou, Anastasios, Speliotis, Thanasis, Petrou, Panagiota, Kakabakos, Sotirios
Format: Article
Language:English
Subjects:
Biomolecules
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biotinylation
Deoxyribonucleic acid
Devices
DNA
DNA - analysis
DNA - genetics
Electrochemistry
Electrodes
Format
Humans
Hybridization
Limit of Detection
Metals - chemistry
Microtechnology - instrumentation
Microtechnology - methods
Mutation
Mutation - genetics
Nucleic Acid Hybridization - methods
Oligonucleotides
Proto-Oncogene Proteins c-ret - genetics
Quantum Dots
Sensors
Streptavidin - metabolism
Transducers
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Summary:A new flexible miniaturized integrated device was microfabricated for the in situ ultrasensitive voltammetric determination of DNA mutation in a microwell format, using quantum dots (QDs) labels. The integrated device consisted of thin Bi, Ag, and Pt films (serving as the working, reference, and counter electrode, respectively) deposited by sputtering on a flexible polyimide substrate. A DNA assay was employed in microwell format, where an immobilized complementary oligonucleotide probe hybridized with the biotinylated target oligonucleotide followed by reaction with streptavidin-conjugated PbS QDs. After the acidic dissolution of the QDs, the flexible sensor was rolled and inserted into the microwell and the Pb­(II) released was determined in situ by anodic stripping voltammetry. Since the analysis took place directly in the microwell, the volume of the working solution was only 100 μL and the target DNA could be detected at a concentration down to 1.1 fmol L–1. The proposed flexible microdevice addresses the restrictions of conventional rigid electrodes while it provides a low cost integrated transducer for the ultrasensitive detection of important biomolecules.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac503791j