Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis

Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is asso...

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Bibliographic Details
Published in:Journal of biophotonics 2015-05, Vol.8 (5), p.408-414
Main Authors: Steelman, Zachary, Meng, Zhaokai, Traverso, Andrew J., Yakovlev, Vladislav V.
Format: Article
Language:English
Subjects:
Bacteria
bacterial
Brillouin zone
Cerebrospinal Fluid Proteins - analysis
Cerebrospinal Fluid Proteins - chemistry
Elevated
Fluid flow
Fluids
Humans
Meningitis
Meningitis, Bacterial - cerebrospinal fluid
molecular
optics and photonics
pathology
Proteins
Spectrometry, Fluorescence
Spectroscopy
Spectrum analysis
Spectrum Analysis - methods
Spectrum Analysis, Raman
Time Factors
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Summary:Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is associated with bacterial meningitis. Brillouin spectroscopy is a widely used optical technique for noninvasive determination of the elastic moduli of materials. We found that elevated protein levels in CSF alter the fluid elasticity sufficiently to be measurable by Brillouin spectroscopy, with model healthy and diseased fluids distinguishable to marked significance (P = 0.014), which increases with sample concentration by dialysis. Typical raw output of a 2‐stage VIPA Brillouin spectrometer: inelastically scattered Brillouin peaks (arrows) and elastically scattered incident radiation (center cross). Brillouin spectroscopy is a noninvasive emerging optical method for the detection of mechanical properties of materials, including fluids. For the first time, we show the feasibility of this technique for biofluid tissue diagnostics. Elevated protein concentrations in mock diseased cerebrospinal fluid are differentiated to a high degree of accuracy, while requiring no sample destruction, modification, or preparation.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201400047