Cep70 regulates microtubule stability by interacting with HDAC6

•We demonstrate that Cep70 enhances microtubule resistance to cold or nocodazole treatment.•Cep70 promotes microtubule stability by regulating tubulin acetylation, and plays an important role in stabilizing microtubules.•Cep70 interacts and colocalizes with histone deacetylase 6 (HDAC6) in the cytop...

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Bibliographic Details
Published in:FEBS letters 2015-07, Vol.589 (15), p.1771-1777
Main Authors: Shi, Xingjuan, Yao, Yanjun, Wang, Yujue, Zhang, Yu, Huang, Qinghai, Zhou, Jun, Liu, Min, Li, Dengwen
Format: Article
Language:English
Subjects:
Acetylation
Base Sequence
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Cycle Proteins - physiology
Cep70
Cold Temperature
HEK293 Cells
HeLa Cells
Histone Deacetylase 6
Histone Deacetylases - metabolism
Humans
Microtubule stability
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Microtubule-Associated Proteins - physiology
Microtubules - metabolism
Nocodazole - pharmacology
Polymerization
Protein Binding
RNA, Small Interfering
Tubulin
Tubulin - metabolism
Tubulin acetylation
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Summary:•We demonstrate that Cep70 enhances microtubule resistance to cold or nocodazole treatment.•Cep70 promotes microtubule stability by regulating tubulin acetylation, and plays an important role in stabilizing microtubules.•Cep70 interacts and colocalizes with histone deacetylase 6 (HDAC6) in the cytoplasm. Microtubules, highly dynamic components of the cytoskeleton, are involved in mitosis, cell migration and intracellular trafficking. Our previous work has shown that the centrosomal protein Cep70 regulates microtubule organization and mitotic spindle orientation in mammalian cells. However, it remains elusive whether Cep70 is implicated in microtubule stability. Here we demonstrate that Cep70 enhances microtubule resistance to cold or nocodazole treatment. Our data further show that Cep70 promotes microtubule stability by regulating tubulin acetylation, and plays an important role in stabilizing microtubules. Mechanistic studies reveal that Cep70 interacts and colocalizes with histone deacetylase 6 (HDAC6) in the cytoplasm. These findings suggest that Cep70 promotes microtubule stability by interaction with HDAC6 and regulation of tubulin acetylation.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2015.06.017