Interactions of Pseudomonas aeruginosa and Corynebacterium spp. with non-phagocytic brain microvascular endothelial cells and phagocytic Acanthamoeba castellanii

Several lines of evidence suggest that Acanthamoeba interact with bacteria, which may aid in pathogenic bacterial transmission to susceptible hosts, and these interactions may have influenced evolution of bacterial pathogenicity. In this study, we tested if Gram-negative Pseudomonas aeruginosa and G...

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Bibliographic Details
Published in:Parasitology research (1987) 2015-06, Vol.114 (6), p.2349-2356
Main Authors: Siddiqui, Ruqaiyyah, Lakhundi, Sahreena, Khan, Naveed Ahmed
Format: Article
Language:English
Subjects:
Acanthamoeba castellanii
Acanthamoeba castellanii - physiology
Amoeba
Amoebas
Animals
Biomedical and Life Sciences
Biomedicine
Brain - blood supply
Corynebacteria
Corynebacterium
Corynebacterium - physiology
Endothelial Cells - physiology
Health aspects
Humans
Immunology
Medical Microbiology
Microbiology
Original Paper
Pseudomonas aeruginosa
Pseudomonas aeruginosa - physiology
Trophozoites
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Summary:Several lines of evidence suggest that Acanthamoeba interact with bacteria, which may aid in pathogenic bacterial transmission to susceptible hosts, and these interactions may have influenced evolution of bacterial pathogenicity. In this study, we tested if Gram-negative Pseudomonas aeruginosa and Gram-positive Corynebacterium spp. can associate/invade and survive inside Acanthamoeba castellanii trophozoites and cysts, as well as non-phagocytic human brain microvascular endothelial cells. The results revealed that both Corynebacterium spp. and P. aeruginosa were able to associate as well as invade and/or taken up by the phagocytic A. castellanii trophozoite. In contrast, P. aeruginosa exhibited higher association as well as invasion of non-phagocytic HBMEC compared with Corynebacterium spp. Notably, P. aeruginosa remained viable during the encystment process and exhibited higher levels of recovery from mature cysts (74.54 bacteria per amoebae) compared with Corynebacterium spp. (2.69 bacteria per amoeba) ( P  
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-015-4432-0