Microbial reduction and precipitation of vanadium (V) in groundwater by immobilized mixed anaerobic culture

• Published in: Bioresource technology 2015-09, Vol.192, p.410-417
• Main Authors: Zhang, Baogang, Hao, Liting, Tian, Caixing, Yuan, Songhu, Feng, Chuanping, Ni, Jinren, Borthwick, Alistair G.L.
• Format: Article
• Language: English
• Subjects: Anaerobic sludge; Anaerobiosis; Biodegradation, Environmental; Biological Oxygen Demand Analysis; Bioreactors - microbiology; Cells, Immobilized - metabolism; Enterobacter - genetics; Enterobacter - metabolism; Groundwater - chemistry; High-Throughput Nucleotide Sequencing; High-throughput pyrosequencing; Hydrogen-Ion Concentration; Lactococcus - genetics; Lactococcus - metabolism; Microbial community diversity; Microbial Consortia - genetics; Microbial Consortia - physiology; Microbial reduction; Phylogeny; Sewage - microbiology; Spirochaeta - genetics; Spirochaeta - metabolism; Vanadium (V); Vanadium - chemistry; Vanadium - metabolism; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - metabolism; Water Purification - instrumentation; Water Purification - methods
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2015.05.102

•Vanadium (V) is effectively reduced by immobilized mixed anaerobic sludge.•V(IV) is the main reduction product and it precipitates instantly from groundwater.•Initial V(V), COD concentrations, pH and conductivity affect the performance.•High-throughput pyrosequencing analysis indicates the decreased diversity.•New functional species as Lactococcus, Enterobacter and Spirochaeta are found. Vanadium is an important contaminant impacted by natural and industrial activities. Vanadium (V) reduction efficiency as high as 87.0% was achieved by employing immobilized mixed anaerobic sludge as inoculated seed within 12h operation, while V(IV) was the main reduction product which precipitated instantly. Increasing initial V(V) concentration resulted in the decrease of V(V) removal efficiency, while this index increased first and then decreased with the increase of initial COD concentration, pH and conductivity. High-throughput 16S rRNA gene pyrosequencing analysis indicated the decreased microbial diversity. V(V) reduction was realized through dissimilatory reduction process by significantly enhanced Lactococcus and Enterobacter with oxidation of lactic and acetic acids from fermentative microorganisms such as the enriched Paludibacter and the newly appeared Acetobacterium, Oscillibacter. This study is helpful to detect new functional species for V(V) reduction and constitutes a step ahead in developing in situ bioremediations of vanadium contamination.