Characterisation of wheat-rye recombinants with RFLP and PCR probes

The introgression of genetic material from alien species into wheat has become an important tool in modern wheat breeding. Ideally, only the trait of interest and no flanking material should be transferred. Random recombination between the genetic material is therefore of paramount importance. In a...

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Bibliographic Details
Published in:Theoretical and applied genetics 1993-02, Vol.85 (8), p.1023-1028
Main Authors: Rogowsky, P.M. (Adelaide Univ., Glen Osmond (Australia). Waite Agricultural Research Inst. Centre for Cereal Biotechnoloy), Sorrells, M.E, Shepherd, K.W, Langridge, P
Format: Article
Language:English
Subjects:
Alien introgression
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Homoeologous recombination
INTROGRESION
INTROGRESSION
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Pteridophyta, spermatophyta
RECOMBINACION
RECOMBINAISON
RFLP marker
SECALE CEREALE
TRITICUM AESTIVUM
Vegetals
Wheat-rye recombinant
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Summary:The introgression of genetic material from alien species into wheat has become an important tool in modern wheat breeding. Ideally, only the trait of interest and no flanking material should be transferred. Random recombination between the genetic material is therefore of paramount importance. In a model system, 17 recombinants putatively between chromosome 1D of wheat and 1R of rye were examined with 60 random RFLP and 3 PCR markers. The recombinants had been generated by removing the normal effect of the Ph1 gene in the wheat background. Amongst the 9 short-arm recombinants, 3 breakpoints were identified but no differentiation could be made between the 5 proximal recombinants. For the 8 long- arm recombinants analysed only 2 breakpoints were identified with 36 markers. However, only a single RFLP marker was able to differentiate between the recombinants. Indeed the long-arm results are consistent with the possibility that only the rye telomeric region had been transferred. These results indicate either a strong clustering of the RFLP markers near the centromere or else imply that recombination induced between wheat and rye in the absence of the normal effect of the Ph1 gene occurs at only restricted sites. The results allow new primary recombinants to be selected for intercrossing to generate secondary recombinants which are expected to have a smaller interstitial rye segment than that present in DR- A1.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00215043