Phospholipase A1-catalyzed hydrolysis of soy phosphatidylcholine to prepare l-α-glycerylphosphorylcholine in organic-aqueous media

• Published in: Food chemistry 2016-01, Vol.190, p.201-206
• Main Authors: Bang, Hyo-Jeong, Kim, In-Hwan, Kim, Byung Hee
• Format: Article
• Language: English
• Subjects: Acyl migration; Catalysis; Glycerylphosphorylcholine - chemistry; Hydrolysis; l-α-Glycerylphosphorylcholine; Phosphatidylcholine; Phosphatidylcholines - chemistry; Phospholipase A1; Phospholipases A1 - chemistry; Response surface methodology; Soy Foods - analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.05.093

•Lecitase Ultra-catalyzed hydrolysis of soy PC to prepare l-α-GPC was modeled with RSM.•Optimal conditions to completely hydrolyze PC to l-α-GPC were established.•Purification methods to separate l-α-GPC from the reaction products were suggested.•The productivity of l-α-GPC was greatly increased using hexane–water biphasic media. This study aimed to optimize the preparation of l-α-glycerylphosphorylcholine (l-α-GPC) via phospholipase A1 (Lecitase Ultra)-catalyzed hydrolysis of soy phosphatidylcholine (PC). The reaction was performed in n-hexane–water biphasic media in a stirred batch reactor, and modeling and optimization were conducted using response surface methodology. Optimal conditions to completely hydrolyze PC to l-α-GPC were: temperature, 50°C; reaction time, 30h; water content, 69g/100g of PC weight; and enzyme loading, 13g/100g of PC weight. The optimal n-hexane-to-water ratio in the medium was 5.8:1 (v/v), and 21.3g of PC was treated as the substrate in 100mL of the medium. l-α-GPC with purity 99.3g/100g was obtained from the reaction products after diethyl ether extraction and silica column chromatography. These findings suggest that the use of n-hexane–water media increases the productivity of l-α-GPC compared to the aqueous media used in enzymatic reaction systems in other published studies.