Super-Resolution Imaging of Plasma Membrane Glycans

Much of the physiology of cells is controlled by the spatial organization of the plasma membrane and the glycosylation patterns of its components, however, studying the distribution, size, and composition of these components remains challenging. A bioorthogonal chemical reporter strategy was used fo...

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Bibliographic Details
Published in:Angewandte Chemie 2014-10, Vol.126 (41), p.11101-11104
Main Authors: Letschert, Sebastian, Göhler, Antonia, Franke, Christian, Bertleff-Zieschang, Nadja, Memmel, Elisabeth, Doose, Sören, Seibel, Jürgen, Sauer, Markus
Format: Article
Language:eng ; ger
Subjects:
Bioorthogonale Chemie
Biotechnology
Chemical compounds
Chemistry
Density
Fluorescence
Glycan
Glycosylation
Hochauflösende Bildgebung
Imaging
Klick-Chemie
Membranes
Membranglykane
Mikroskopie
Plasma
Precursors
Strategy
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Summary:Much of the physiology of cells is controlled by the spatial organization of the plasma membrane and the glycosylation patterns of its components, however, studying the distribution, size, and composition of these components remains challenging. A bioorthogonal chemical reporter strategy was used for the efficient and specific labeling of membrane‐associated glycoconjugates with modified monosaccharide precursors and organic fluorophores. Super‐resolution fluorescence imaging was used to visualize plasma membrane glycans with single‐molecule sensitivity. Our results demonstrate a homogeneous distribution of N‐acetylmannosamine (ManNAc)‐, N‐acetylgalactosamine (GalNAc)‐, and O‐linked N‐acetylglucosamine (O‐GlcNAc)‐modified plasma membrane proteins in different cell lines with densities of several million glycans on each cell surface. Glykan‐Kartierung: Klick‐Chemie und hochauflösende Bildgebung visualisieren und quantifizieren ManNAc‐, GalNAc‐ und O‐GlcNAc‐modifizierte Glykane in der Plasmamembran von Säugerzellen mit Einzelmolekülempfindlichkeit (siehe Bild). Eine einzige Zelle kann demnach über zehn Millionen Glykane enthalten; diese sind gleichmäßig verteilt und bilden weder Cluster noch Nanodomänen.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201406045