Ammonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) Desv

• Published in: In vitro cellular & developmental biology. Plant 1995-10, Vol.31 (4), p.211-214
• Main Authors: Kwa, S.H. (National University of Singapore, Singapore.), Wee, Y.C, Kumar, P.P
• Format: Article
• Language: English
• Subjects: 2,4-D; ABSORCION DE SUSTANCIAS NUTRITIVAS; ABSORPTION DE SUBSTANCES NUTRITIVES; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AMMONIA; AMMONIAC; AMONIACO; Biological and medical sciences; Biotechnology; CAL; CALLO; CALLUS; CARBON DIOXIDE; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE MEDIA; DIOXIDO DE CARBONO; DIOXYDE DE CARBONE; EFECTO INVERNADERO; EFFET DE SERRE; ENZYMIC ACTIVITY; Eukaryotic cell cultures; FERNS; FOUGERE; Fundamental and applied biological sciences. Psychology; GREENHOUSE EFFECT; HELECHOS; In vitro propagation: entire plant regeneration from tissues and cell cultures; MEDIO DE CULTIVO; Methods. Procedures. Technologies; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; NITRATE; NITRATE REDUCTASE; NITRATES; NITRATO REDUCTASA; NITRATOS; NUTRIENT UPTAKE; Plant cells and fungal cells; Plant physiology and development; SACCHAROSE; SUCROSA; SUCROSE; TISSUE CULTURE; Tissue cultures, protoplasts
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/BF02632024

The uptake of nitrate and ammonium by callus of Platycerium coronarium from the culture medium was examined. Nitrate reductase activity of photoautotrophic callus cultures under CO sub(2) enrichment was significantly lower compared to the cultures without CO sub(2) enrichment, but higher than that of heterotrophic callus cultured on medium with 2% (wt/vol) sucrose. When sucrose concentration of the heterotrophic culture was lowered to 0.2%, nitrate reductase activity increased. The level of nitrate reductase activity increased by about 25% in the heterotrophic callus with an increase in 2,4-D from 2 mu M to 10 mu M, despite a decline in fresh weight gain. However, photoautotrophic cultures with 1% CO sub(2) enrichment showed 20% decline in nitrate reductase activity and 45% decline in fresh weight gain with a similar increase in 2,4-D level. The rate of uptake of nitrate from the culture medium was unrelated to the level of nitrate reductase activity in the callus. For photoautotrophic callus under CO sub(2) enrichment, the presence of 1% (vol/vol) CO sub(2) generally resulted in the highest rate of nitrate uptake. The rate of uptake of ammonium was higher for callus cultured on 2 mu M 2,4-D compared to that on 10 mu M 2,4-D.