Purification, characterization, and biological activities of broccolini lectin

Plant lectins have displayed a variety of biological activities. In this study, for the first time, a 27 kDa arabinose‐ and mannose‐specific lectin from Broccolini (Brassica oleracea Italica × Alboglabra), named as BL (Broccolini lectin), was purified by an activity‐driven protocol. Mass spectrometr...

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Bibliographic Details
Published in:Biotechnology progress 2015-05, Vol.31 (3), p.736-743
Main Authors: Xu, Pingping, Zhang, Ting, Guo, Xiaolei, Ma, Chungwah, Zhang, Xuewu
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
antibacteria
Antineoplastic Agents - pharmacology
antitumor
brassica
Brassica - chemistry
Brassica oleracea italica
broccolini
Cell Line, Tumor
Chemical Phenomena
Chickens
Dose-Response Relationship, Drug
Erythrocytes - drug effects
Escherichia coli - drug effects
Helicobacter pylori - drug effects
Hemagglutination - drug effects
Hep G2 Cells
Humans
Hydrogen-Ion Concentration
Inhibitory Concentration 50
lectin
Lectins - pharmacology
MCF-7 Cells
Plant Extracts - pharmacology
Pseudomonas aeruginosa - drug effects
purification
Shigella dysenteriae - drug effects
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staphylococcus aureus - drug effects
Temperature
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Summary:Plant lectins have displayed a variety of biological activities. In this study, for the first time, a 27 kDa arabinose‐ and mannose‐specific lectin from Broccolini (Brassica oleracea Italica × Alboglabra), named as BL (Broccolini lectin), was purified by an activity‐driven protocol. Mass spectrometry analysis and database search indicated that no matches with any plant lectin were found, but BL contained some peptide fragments (QQQGQQGQQLQQVISR, QQGQQQGQQGQQLQQVISR and VCNIPQVSVCPF QK). BL exhibited hemagglutinating activity against chicken erythrocytes at 4 µg/mL. BL retained full hemagglutinating activity at pH 7–8 and temperature 30–40°C, and had an optimal activity in Ca2+ solution. Bioactivity assay revealed that BL exhibited dose‐dependent inhibition activity on 5 bacterial species with IC50 values of 143.95–486.33 μg/mL, and on 3 cancer cells with IC50 values of 178.82–350.93 μg/mL. Notably, 5‐fold reduction in IC50 values was observed on normal L‐O2 vs cancerous HepG‐2 cells (924.35 vs. 178.82 μg/mL). This suggests that BL should be promising in food and medicine. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:736–743, 2015
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.2070