Thermo-chemotherapy Induced miR-218 upregulation inhibits the invasion of gastric cancer via targeting Gli2 and E-cadherin

Thermo-chemotherapy has been proven to reduce the invasion capability of cancer cells. However, the molecular mechanism underlying this anti-invasion effect is still unclear. In this study, the role of thermo-chemotherapy in the inhibition of tumor invasion was studied. The results demonstrated that...

Full description

Saved in:
Bibliographic Details
Published in:Tumor biology 2015-08, Vol.36 (8), p.5807-5814
Main Authors: Ruan, Qiang, Fang, Zhi-yuan, Cui, Shu-zhong, Zhang, Xiang-liang, Wu, Yin-bing, Tang, Hong-sheng, Tu, Yi-nuo, Ding, Yan
Format: Article
Language:English
Subjects:
Adult
Aged
Biomedical and Life Sciences
Biomedicine
Cadherins - genetics
Cancer Research
Cell adhesion & migration
Cell Proliferation - drug effects
Chemotherapy
Clinical outcomes
Epithelial-Mesenchymal Transition - drug effects
Female
Gastric cancer
Gene Expression Regulation, Neoplastic - drug effects
Humans
Hyperthermia, Induced
Kruppel-Like Transcription Factors - genetics
Lymphatic Metastasis
Male
MicroRNAs - biosynthesis
MicroRNAs - genetics
Middle Aged
Neoplasm Invasiveness - genetics
Nuclear Proteins - genetics
Protein expression
Research Article
Stomach Neoplasms - drug therapy
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Zinc Finger Protein Gli2
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thermo-chemotherapy has been proven to reduce the invasion capability of cancer cells. However, the molecular mechanism underlying this anti-invasion effect is still unclear. In this study, the role of thermo-chemotherapy in the inhibition of tumor invasion was studied. The results demonstrated that expression of miR-218 was downregulated in gastric cancer tissues, which had a positive correlation with tumor invasion and metastasis. In vitro thermo-chemotherapy increased miR-218 expression in SGC7901 cells and inhibited both proliferation and invasion of cancer cells. Gli2 was identified as a downstream target of miR-218, and its expression was negatively regulated by miR-218. The thermo-chemotherapy induced miR-218 upregulation was also accompanied by increasing of E-cadherin expression. In conclusion, the present study indicates that thermo-chemotherapy can effectively decrease the invasion capability of cancer cells and increase cell-cell adhesion. miR-218 and its downstream target Gli2, as well as E-cadherin, participate in the anti-invasion process.
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-015-3250-4