Enzymatic hydrolysis of ovomucoid and the functional properties of its hydrolysates

Ovomucoid is well known as a “trypsin inhibitor” and is considered to be the main food allergen in egg. However, the negative functions of ovomucoid can be eliminated if the protein is cut into small peptides. The objectives of this study were to hydrolyze ovomucoid using various enzyme combinations...

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Bibliographic Details
Published in:Poultry science 2015-09, Vol.94 (9), p.2280-2287
Main Authors: Abeyrathne, E. D. N. S., Lee, H. Y., Jo, C., Suh, J. W., Ahn, D. U.
Format: Article
Language:English
Subjects:
Angiotensin-Converting Enzyme Inhibitors - analysis
Animals
Antioxidants - analysis
Avian Proteins - chemistry
Avian Proteins - metabolism
Chelating Agents - analysis
Chickens - metabolism
Electrophoresis, Polyacrylamide Gel
Endopeptidases - chemistry
Endopeptidases - metabolism
Hydrolysis
Metals - chemistry
Ovomucin - chemistry
Ovomucin - metabolism
Ovum - chemistry
Peptides - chemistry
Peptides - metabolism
Tandem Mass Spectrometry
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Summary:Ovomucoid is well known as a “trypsin inhibitor” and is considered to be the main food allergen in egg. However, the negative functions of ovomucoid can be eliminated if the protein is cut into small peptides. The objectives of this study were to hydrolyze ovomucoid using various enzyme combinations, and compare the functional properties of the hydrolysates. Purified ovomucoid was dissolved in distilled water (20 mg/mL) and treated with 1% of pepsin, α-chymotrypsin, papain, and alcalase, singly or in combinations. Sodium sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) results of the hydrolysates indicated that pepsin (OMP), alcalase (OMAl), alcalase + trypsin (OMAlTr), and alcalase + papain (OMAlPa) treatments best hydrolyzed the ovomucoid, and the 4 treatments were selected to determine their functional characteristics. Among the 4 enzyme treatments, hydrolysate from OMAlTr showed the highest iron-chelating and antioxidant activities, while OMP showed higher ACE-inhibitory activity, but lower Fe-chelating activity than the other treatments. However, no difference in the copper-chelating activity among the treatments was found. MS/MS analysis identified numerous peptides from the hydrolysates of OMAlPa and OMAlTr, and majority of the peptides produced were
ISSN:0032-5791
1525-3171
DOI:10.3382/ps/pev196