Contrast-enhanced molecular ultrasound differentiates endoglin genotypes in mouse embryos

Targeted ultrasound contrast imaging has the potential to become a reliable molecular imaging tool. A better understanding of the quantitative aspects of molecular ultrasound technology could facilitate the translation of this technique to the clinic for the purposes of assessing vascular pathology...

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Bibliographic Details
Published in:Angiogenesis (London) 2015-01, Vol.18 (1), p.69-81
Main Authors: Denbeigh, J. M., Nixon, B. A., Lee, J. J. Y., Jerkic, M., Marsden, P. A., Letarte, M., Puri, M. C., Foster, F. S.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Blotting, Western
Cancer Research
Cardiology
Cell Adhesion - physiology
Cell Biology
Embryo, Mammalian - diagnostic imaging
Endoglin
Endothelial Cells - diagnostic imaging
Endothelial Cells - physiology
Genotype
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - physiology
Lymphocytes, Null
Mice
Mice, Knockout
Microbubbles
Molecular Imaging
Oncology
Ophthalmology
Original Paper
Rats
Ultrasonography
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Summary:Targeted ultrasound contrast imaging has the potential to become a reliable molecular imaging tool. A better understanding of the quantitative aspects of molecular ultrasound technology could facilitate the translation of this technique to the clinic for the purposes of assessing vascular pathology and detecting individual response to treatment. The objective of this study was to evaluate whether targeted ultrasound contrast-enhanced imaging can provide a quantitative measure of endogenous biomarkers. Endoglin, an endothelial biomarker involved in the processes of development, vascular homeostasis, and altered in diseases, including hereditary hemorrhagic telangiectasia type 1 and tumor angiogenesis, was the selected target. We used a parallel plate perfusion chamber in which endoglin-targeted (MB E ), rat isotype IgG 2 control and untargeted microbubbles were perfused across endoglin wild-type ( Eng +/+ ), heterozygous ( Eng +/− ) and null ( Eng −/− ) embryonic mouse endothelial cells and their adhesion quantified. Microbubble binding was also assessed in late-gestation, isolated living transgenic Eng +/− and Eng +/+ embryos. Nonlinear contrast-specific ultrasound imaging performed at 21 MHz was used to collect contrast mean power ratios for all bubble types. Statistically significant differences in microbubble binding were found across genotypes for both in vitro ( p  
ISSN:0969-6970
1573-7209
DOI:10.1007/s10456-014-9447-0