Scaffold Effects on Osteogenic Differentiation of Equine Mesenchymal Stem Cells: An In Vitro Comparative Study

The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen‐based scaffolds or with fibrin glue. The results indicat...

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Bibliographic Details
Published in:Macromolecular bioscience 2013-03, Vol.13 (3), p.348-355
Main Authors: Nino-Fong, Rodolfo, McDuffee, Laurie A., Esparza Gonzalez, Blanca P., Kumar, M. Ramesh, Merschrod S., Erika F., Poduska, Kristin M.
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
Biocompatibility
Biomedical materials
Bone marrow
Bone Marrow Cells - cytology
Cell Differentiation - drug effects
Cell Survival - drug effects
Cells, Cultured
collagen
Collagen - chemistry
Collagen - pharmacology
Differentiation
Fibrin Tissue Adhesive - chemistry
Fibrin Tissue Adhesive - pharmacology
Horses
hydrogels
In vitro testing
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Muscle, Skeletal - cytology
Osteocytes - cytology
Osteocytes - drug effects
Periosteum - cytology
Protein Isoforms - chemistry
Protein Isoforms - pharmacology
Regenerative
Scaffolds
Stem cells
Tissue Engineering - methods
Tissue Scaffolds
Viability
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Summary:The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen‐based scaffolds or with fibrin glue. The results indicate that bone marrow cells are the best source of MSCs for osteogenic differentiation, and that an electrochemically aggregated collagen gives the highest cell viability and best osteogenic differentiation among the four kinds of scaffolds studied. Stem‐cell‐based therapy is an important strategy in the treatment of organ and tissue injuries. This study indicates that a collagen‐based scaffold supports osteogenic differentiation in vitro, promising results for possible treatments in regenerative medicine.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201200355