Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes

• Published in: Chinese journal of oceanology and limnology 2015-07, Vol.33 (4), p.913-918
• Main Author: 李艳宇 张涛 张丛尧 朱莹 丁鉴锋 马悦欣
• Format: Article
• Language: English
• Subjects: Acinetobacter; Bacillus; Bacteria; bacterial communities; Biodiversity; Biology; Deoxyribonucleic acid; DNA; DNA测序; Earth and Environmental Science; Earth Sciences; Enterobacter; Exiguobacterium; Firmicutes; Fish; intestinal microorganisms; intestines; Marine; Marine biology; mucus; Oceanography; physiology; polymerase chain reaction; Proteobacteria; Pseudoalteromonas; Pseudomonas; ribosomal DNA; sampling; Shewanella; Staphylococcus; Takifugu rubripes; Vibrio; 养殖; 河豚鱼; 细菌培养; 细菌多样性; 聚合酶链反应; 肠道粘膜; 金黄色葡萄球菌
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-015-4219-2

The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16S rDNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216E medium. All the isolates were classified into two phyla-Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota （68,3%）. At the genus level, Vibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and Shewanella were isolated from the intestine, with representatives of the genera Vibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.