In vitro assessment of three dimensional dense chitosan-based structures to be used as bioabsorbable implants

Chitosan biocompatibility and biodegradability properties make this biopolymer promising for the development of advanced internal fixation devices for orthopedic applications. This work presents a detailed study on the production and characterization of three dimensional (3D) dense, non-porous, chit...

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Bibliographic Details
Published in:Journal of the mechanical behavior of biomedical materials 2014-12, Vol.40, p.413-425
Main Authors: Guitian Oliveira, Nuno, Sirgado, Tatiana, Reis, Luís, Pinto, Luís F V, da Silva, Cláudia Lobato, Ferreira, Frederico Castelo, Rodrigues, Alexandra
Format: Article
Language:English
Subjects:
Absorbable Implants
Biocompatible Materials - chemistry
Cell Adhesion
Cell Proliferation
Chitosan
Chitosan - chemistry
Compressive strength
Glycerols
Humans
In Vitro Techniques
In vitro testing
Magnetic Resonance Spectroscopy
Mechanical properties
Mesenchymal Stromal Cells - cytology
Molecular structure
Surgical implants
Three dimensional
Tissue Engineering
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Summary:Chitosan biocompatibility and biodegradability properties make this biopolymer promising for the development of advanced internal fixation devices for orthopedic applications. This work presents a detailed study on the production and characterization of three dimensional (3D) dense, non-porous, chitosan-based structures, with the ability to be processed in different shapes, and also with high strength and stiffness. Such features are crucial for the application of such 3D structures as bioabsorbable implantable devices. The influence of chitosan's molecular weight and the addition of one plasticizer (glycerol) on 3D dense chitosan-based products' biomechanical properties were explored. Several specimens were produced and in vitro studies were performed in order to assess the cytotoxicity of these specimens and their physical behavior throughout the enzymatic degradation experiments. The results point out that glycerol does not impact on cytotoxicity and has a high impact in improving mechanical properties, both elasticity and compressive strength. In addition, human mesenchymal stem/stromal cells (MSC) were used as an ex-vivo model to study cell adhesion and proliferation on these structures, showing promising results with fold increase values in total cell number similar to the ones obtained in standard cell culture flasks.
ISSN:1751-6161
1878-0180
DOI:10.1016/j.jmbbm.2014.09.014