Oil extraction from algae: A comparative approach

• Published in: Biotechnology and applied biochemistry 2015-05, Vol.62 (3), p.375-382
• Main Authors: Valizadeh Derakhshan, Mehrab, Nasernejad, Bahram, Abbaspour-Aghdam, Farzin, Hamidi, Mohammad
• Format: Article
• Language: English
• Subjects: Algae; biodiesel; Biofuels; Chemical Fractionation - methods; Chlorella vulgaris; Chlorella vulgaris - chemistry; Chloroform; Ethanol; Ethyl alcohol; Extraction; Fatty acids; Lipids; Methanol; Moisture content; oil extraction; optimization; Plant Oils - isolation & purification; sonication; Sonication - methods; Ultrasonic testing; Water content
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1002/bab.1270

In this article, various methods including soxhlet, Bligh & Dyer (B&D), and ultrasonic‐assisted B&D were investigated for the extraction of lipid from algal species Chlorella vulgaris. Relative polarity/water content and impolar per polar ratios of solvents were considered to optimize the relative proportions of each triplicate agent by applying the response surface method (RSM). It was found that for soxhlet, hexane–methanol (54–46%, respectively) with total lipid extraction of 14.65% and chloroform–methanol (54–46%, respectively) with the extraction of 19.87% lipid were the best set of triplicate where further addition of acetone to the first group and ethanol to the second group did not contributed to further extraction. In B&D, however, chloroform–methanol–water (50%–35%–15%, respectively) reached the all‐time maximum of 24%. Osmotic shock as well as ultrasonication contributed to 3.52% of further extraction, which is considered to promote the total yield up to almost 15%. From the growth data and fatty acid analysis, the applied method was assessed to be appropriate for biodiesel production with regard to selectivity and extraction yield.