ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes

• Published in: Molecular immunology 2015-10, Vol.67 (2), p.206-212
• Main Authors: Zhang, Hong, Wang, Wen-chen, Chen, Jia-kuan, Zhou, Lin, Wang, Ming, Wang, Zhen-dong, Yang, Bo, Xia, Yan-ming, Lei, Shi, Fu, En-qing, Jiang, Tao
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; Acute lung injury; Acute Lung Injury - genetics; Acute Lung Injury - pathology; Animals; Down-Regulation - genetics; Endonucleases; Inflammation; Inflammation - genetics; Inflammation - pathology; Inflammation Mediators - metabolism; Lipopolysaccharides; Luciferases - metabolism; Lung - pathology; Male; Mice, Inbred BALB C; Models, Biological; mRNA stability; Proteins - metabolism; Proto-Oncogene Proteins c-fos - genetics; RNA Stability - genetics; Tumor Suppressor Proteins - metabolism; ZC3H12D
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2015.05.018

•Zc3h12d was downregulated in the immune cells of lung and airways during LPS-induced acute lung injury.•ZC3H12D level was negatively correlated with the expression levels of proinflammatory genes.•ZC3H12D destabilized the mRNA of c-fos, NF-κB, and cytokines including IL-1β, IL-6, and TNF-α.•ZC3H12D might regulate initial inflammation surge and chronic inflammation upon endotoxin exposure. Infection in airspaces and lung parenchyma may cause acute lung injury and multiple organ dysfunction syndrome due to acute inflammatory response, leading to organ failure and high mortality. ZC3H12D has been shown to modulate Toll-like receptor signaling. This study aimed to investigate the change of ZC3H12D during acute lung injury and its role in inflammation processes. Mice were challenged with lipopolysaccharides (LPS) intratracheally. The expression levels of Zc3h12d, NF-κB, and cytokines were analyzed by quantitative real-time PCR (qPCR), ELISA, and Western blot. The mRNA stability was assessed by qPCR after cells were treated with actinomycin D for specified times. The 3′ untranslated region (3′-UTR) of c-fos was cloned immediately downstream of the luciferase coding sequence driven by CMV promoter and luciferase activity was measured with a Luciferase Assay kit. Upon LPS treatment, ZC3H12D levels were reduced in mouse immune cells, whereas levels of NF-κB, IL-6, and TNF-α were significantly increased. Knockdown Zc3h12d in THP1 cells resulted in the upregulation of NF-κB while overexpression of Zc3h12d inhibited NF-κB expression. Ectopic Zc3h12d significantly reduced the mRNA stability of c-fos, NF-κB, TNF-α, IL-1β, and IL-6. Attachment of the c-fos 3′-UTR made luciferase expression levels sensitive to levels of ZC3H12D. The data indicated that ZC3H12D could suppress both the initial inflammation storm and chronic inflammation by targeting the mRNA of cytokines as well as NF-κB and c-fos.