Assignment of five porcine genes by pig-mink cell hybrids to pig chromosomes 2, 5, 8, 12

• Published in: Mammalian genome 1998-11, Vol.9 (11), p.913-914
• Main Authors: Koroleva, I V, Malchenko, S N, Shukri, N M, Ivanova, E V, Kuznetsov, S B, Zhdanova, N S, Bendixen, C
• Format: Article
• Language: English
• Subjects: Animals; Chromosome Mapping; Chromosomes; DNA-Binding Proteins - genetics; Hybrid Cells; Mink; Molecular Sequence Data; Neurofibromatosis 1 - genetics; Polymerase Chain Reaction; Receptor, Fibroblast Growth Factor, Type 4; Receptors, Fibroblast Growth Factor - genetics; Receptors, Mineralocorticoid - genetics; Swine - genetics
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/s003359900896

Comparative gene mapping makes it possible to identify homologous chromosome segments in distantly related mammals and to analyze genome organization and evolution. An important aspect of such information is to allow the exchange of information between species. Expressed genes are suitable landmarks for comparative mapping. Lyons and coworkers (1997) designed evolutionarily conserved primer pair sequences specific for anchor locus gene amplification. Comparative anchor tagged sequences (CATS) were designed to include both part of an exon for verification of sequence homology of PCR products and a part of an intron for detection of intraspecies polymorphism. Here we describe the assignment of five porcine genes to pig chromosomes, using a set of pig-mink hybrids. Four genes, fibroblast growth factor receptor 4 (FGFR4), mineralocorticoid receptor (MLR), neurofibromatosis 1 (NF1), and EV12 protein gene (EV12A), have been mapped by PCR to Chrs 2, 9, 12, and 12 respectively by use of CATS primers. The recombination and repair gene (RAD52) was localized on pig chromosome (Chr) 5 by Southern blot analysis with a heterologous cDNA clone from mouse as a probe.