Mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production

• Published in: Ultrasonics sonochemistry 2016-01, Vol.28, p.207-217
• Main Authors: Ramadoss, Govindarajan, Muthukumar, Karuppan
• Format: Article
• Language: English
• Subjects: Bioethanol; Biofuels - microbiology; Catalysis; Cellulose - chemistry; Cellulose - metabolism; Delignification; Ethanol - chemistry; Fermentation; Holocellulose recovery; Hydrogen Peroxide - chemistry; Hydrolysis; Metal salt; Metals - chemistry; Saccharomyces cerevisiae - metabolism; Saccharum - chemistry; Salts - chemistry; Sonication; Sugarcane bagasse; Temperature; Ultrasound
• ISSN: 1350-4177; 1873-2828
• DOI: 10.1016/j.ultsonch.2015.07.006

•Ultrasound assisted metal salt pretreatment of sugarcane bagasse was studied.•Mechanism of UATP was explored.•Degraded lignin products were analyzed.•The maximum theoretical yield of glucose obtained was 87.8%. This study presents the ultrasound assisted pretreatment of sugarcane bagasse (SCB) using metal salt with hydrogen peroxide for bioethanol production. Among the different metal salts used, maximum holocellulose recovery and delignification were achieved with ultrasound assisted titanium dioxide (TiO2) pretreatment (UATP) system. At optimum conditions (1% H2O2, 4g SCB dosage, 60min sonication time, 2:100M ratio of metal salt and H2O2, 75°C, 50% ultrasound amplitude and 70% ultrasound duty cycle), 94.98±1.11% holocellulose recovery and 78.72±0.86% delignification were observed. The pretreated SCB was subjected to dilute acid hydrolysis using 0.25% H2SO4 and maximum xylose, glucose and arabinose concentration obtained were 10.94±0.35g/L, 14.86±0.12g/L and 2.52±0.27g/L, respectively. The inhibitors production was found to be very less (0.93±0.11g/L furfural and 0.76±0.62g/L acetic acid) and the maximum theoretical yield of glucose and hemicellulose conversion attained were 85.8% and 77%, respectively. The fermentation was carried out using Saccharomyces cerevisiae and at the end of 72h, 0.468g bioethanol/g holocellulose was achieved. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis of pretreated SCB was made and its morphology was studied using scanning electron microscopy (SEM). The compounds formed during the pretreatment were identified using gas chromatography–mass spectrometry (GC–MS) analysis.