Identification and characterization of an injury-induced skeletal progenitor

The postnatal skeleton undergoes growth, remodeling, and repair. We hypothesized that skeletal progenitor cells active during these disparate phases are genetically and phenotypically distinct. We identified a highly potent regenerative cell type that we term the fracture-induced bone, cartilage, st...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2015-08, Vol.112 (32), p.9920-9925
Main Authors: Marecic, Owen, Tevlin, Ruth, McArdle, Adrian, Seo, Eun Young, Wearda, Taylor, Duldulao, Christopher, Walmsley, Graham G., Nguyen, Allison, Weissman, Irving L., Chan, Charles K. F., Longaker, Michael T.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biological Sciences
Bone and Bones - pathology
Bone Development
Bony Callus - cytology
Cartilage - pathology
Cell Proliferation
Cell Separation
Cells
Femur - pathology
Fractures, Bone - pathology
Gene expression
Gene Expression Profiling
Genotype & phenotype
Hindlimb - radiation effects
Integrin alpha6 - metabolism
Male
Mice, Inbred C57BL
Osteogenesis
Phenotype
Rodents
Skeletal system
Stem Cells - cytology
Stromal Cells - cytology
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Summary:The postnatal skeleton undergoes growth, remodeling, and repair. We hypothesized that skeletal progenitor cells active during these disparate phases are genetically and phenotypically distinct. We identified a highly potent regenerative cell type that we term the fracture-induced bone, cartilage, stromal progenitor (f-BCSP) in the fracture callus of adult mice. Thef-BCSP possesses significantly enhanced skeletogenic potential compared with BCSPs harvested from uninjured bone. It also recapitulates many gene expression patterns involved in perinatal skeletogenesis. Our results indicate that the skeletal progenitor population is functionally stratified, containing distinct subsets responsible for growth, regeneration, and repair. Furthermore, our findings suggest that injury-induced changes to the skeletal stem and progenitor microenvironments could activate these cells and enhance their regenerative potential.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1513066112