Volatile anaesthetic halothane causes DNA damage in A549 lung cells

The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane. The results obtained from the comet assay clearly show that halothane, applied at 3.0 mM con...

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Bibliographic Details
Published in:Toxicology (Amsterdam) 2006-08, Vol.20 (5), p.585-593
Main Authors: Topouzova-Hristova, T., Daza, P., Garcia-Herdugo, G., Stephanova, E.
Format: Article
Language:English
Subjects:
Anesthetics, Inhalation - toxicity
Cell Line, Tumor
Cell morphology
Cell Nucleus - drug effects
Comet assay
DNA Damage - drug effects
DNA integrity
Halothane
Halothane - toxicity
Humans
Lung - drug effects
Lung - ultrastructure
Lung cells
Micronuclei, Chromosome-Defective
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Summary:The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane. The results obtained from the comet assay clearly show that halothane, applied at 3.0 mM concentration, causes DNA and cell damage. Cells exhibited nuclear fragmentation and budding early after treatment and these events gradually increased during the next few days. The presence of a large number of mini-comets after single cell gel electrophoresis was found to represent apoptotic bodies with fragmented DNA. Our results demonstrate apoptosis-like changes after in vitro exposure of A549 cells to the volatile anaesthetic halothane. The majority of the affected cells did not recover and were directed to cell death.
ISSN:0887-2333
0300-483X
1879-3177
DOI:10.1016/j.tiv.2005.10.004