Organ distribution of red sea bream iridovirus (RSIV) DNA in asymptomatic yearling and fingerling rock bream ( Oplegnathus fasciatus) and effects of water temperature on transition of RSIV into acute phase

Most diagnostic studies of red sea bream iridovirus (RSIV) disease in fish have been conducted on the acutely infected fish, and spleen has been used commonly as a target organ for diagnosis. However, little information about persistent RSIV infection is available, and the distribution of RSIV in or...

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Bibliographic Details
Published in:Aquaculture 2006-06, Vol.256 (1), p.23-26
Main Authors: Kyung Choi, Su, Ryun Kwon, Se, Kwon Nam, Yoon, Koo Kim, Sung, Hong Kim, Ki
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal aquaculture
Animal diseases
Animal productions
Aquaculture
Biological and medical sciences
bream
carrier state
disease detection
fingerlings
Fish
fish culture
fish diseases
Fundamental and applied biological sciences. Psychology
General aspects
Infections
Iridovirus
mariculture
Marine
marine fish
Oplegnathus fasciatus
Organ distribution
Perciformes
Persistent infection
Rock bream
RSIV
Spleen
Temperature
Temperature effects
tissue distribution
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
viral diseases of animals and humans
water temperature
yearlings
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Summary:Most diagnostic studies of red sea bream iridovirus (RSIV) disease in fish have been conducted on the acutely infected fish, and spleen has been used commonly as a target organ for diagnosis. However, little information about persistent RSIV infection is available, and the distribution of RSIV in organs and tissues in persistently infected fish has not been systematically determined. In the present study, organ distribution of RSIV in asymptomatic carriers of yearling and fingerling rock bream was analyzed using PCR, and transition possibility of RSIV into acute phase in both age groups by elevating water temperature was investigated. RSIV DNA was predominantly detected in heart, stomach, intestine, muscle, eye and gill by nested-PCR from asymptomatic yearling rock bream. Strikingly, the detection frequency of RSIV DNA in spleen was very low. In fingerling rock bream, consistent with the result of yearling, the frequency of RSIV DNA detection in spleen was low. Fingerling rock bream showed a high cumulative mortality by water temperature elevation, and RSIV DNA was detected by primary PCR from dead fish. In yearling rock bream, however, there were no mortality and no detection of RSIV DNA by primary PCR during water temperature elevation period.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2006.01.026