Development of the pancreas in medaka, Oryzias latipes, from embryo to adult

• Published in: Development, growth & differentiation growth & differentiation, 2015-10, Vol.57 (8), p.557-569
• Main Authors: Otsuka, Takayoshi, Tsukahara, Tatsuya, Takeda, Hiroyuki
• Format: Article
• Language: English
• Subjects: Animals; Gene Expression Regulation, Developmental; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; islet; medaka; Organogenesis - genetics; Organogenesis - physiology; Oryzias - embryology; Oryzias - metabolism; pancreas; Pancreas - embryology; Pancreas - metabolism; pdx1; ptf1a; Trans-Activators - genetics; Trans-Activators - metabolism
• ISSN: 0012-1592; 1440-169X
• DOI: 10.1111/dgd.12237

To address conserved and unique features of fish pancreas development, we performed extensive analyses of pancreatic development in medaka embryos and adults using pdx1‐ and ptf1a‐transgenic medaka, in situ hybridization and immunohistochemistry. The markers used in these analyses included pdx1, nkx6.1, nkx6.2, nkx2.2, Islet1, insulin, Somatostatin, glucagon, ptf1a, ela3l, trypsin, and amylase. The double transgenic (Tg) fish produced in the present study visualizes the development of endocrine (pdx1+) and exocrine (ptf1a+) parts simultaneously in living fishes. Like other vertebrates, the medaka pancreas develops as two (dorsal and ventral) buds in the anterior gut tube, which soon fuse into a single anlagen. The double Tg fish demonstrates that the differential property between the two buds is already established at the initial phase of bud development as indicated by strong pdx1 expression in the dorsal one. This Tg fish also allowed us to examine the gross morphology and the structure of adult pancreas and revealed unique characters of medaka pancreas such as broad and multiple connections with the gut tube along the anterior–posterior axis. This article is the endocrine component of principal and secondary islets. Insulin‐producing cells and Somatostatin‐producing cells are located in the central of islets and glucagon‐producing cells are in the periphery.