Comparison of three media for transport and storage of the samples collected for detection of avian influenza virus

•We compared three media for transport and storage of samples for virus detection.•We found PBS–glycerol is superior to PBS and BHI broth as the media.•The data are useful to optimize detection of avian influenza viruses. Detection of avian influenza viruses (AIVs) is important for diagnosis, survei...

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Bibliographic Details
Published in:Journal of virological methods 2015-09, Vol.222, p.202-205
Main Authors: Zhang, Xiao-Chun, Liu, Shuo, Hou, Guang-Yu, Zhuang, Qing-Ye, Wang, Kai-Cheng, Jiang, Wen-Ming, Wang, Su-Chun, Li, Jin-Ping, Yu, Jian-Min, Du, Xiang, Huang, Bao-Xu, Chen, Ji-Ming
Format: Article
Language:English
Subjects:
Animals
Avian influenza virus
Birds
Buffers
Culture Media - chemistry
Detection
Influenza A virus - isolation & purification
Influenza in Birds - diagnosis
Influenza in Birds - virology
Medium
Microbial Viability
Poultry
Sample
Specimen Handling - methods
Storage
Temperature
Time Factors
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Summary:•We compared three media for transport and storage of samples for virus detection.•We found PBS–glycerol is superior to PBS and BHI broth as the media.•The data are useful to optimize detection of avian influenza viruses. Detection of avian influenza viruses (AIVs) is important for diagnosis, surveillance and control of avian influenza which is of great economic and public health significance. Proper transport and storage of samples is critical for the detection when the samples cannot be detected immediately. As recommended by some international or national authoritative entities and some publications, phosphate buffered saline (PBS), PBS–glycerol and brain heart infusion broth (BHIB) are frequently used for transport and storage of the samples collected for detection of AIVs worldwide. In this study, we compared these three media for transport and storage of simulated and authentic swab and feces samples collected for detection of AIVs using virus isolation and reverse transcription-PCR. The results suggest that PBS–glycerol is superior to PBS and BHIB as the sample transport and storage media. The results also suggest that the samples collected for detection of AIVs should be detected as soon as possible because the virus concentration of the samples may decline rapidly during storage within days at 4 or −20°C.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2015.07.001