Activation and function of murine primary microglia in the absence of the prion protein

Abstract The prion protein (PrPC ) is predominantly expressed in the nervous and immune systems and is involved in relevant cell signaling. Microglia participate in neuroimmune interactions, and their regulatory mechanisms are critical for both health and disease. Despite recent reports with a micro...

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Bibliographic Details
Published in:Journal of neuroimmunology 2015-09, Vol.286, p.25-32
Main Authors: Pinheiro, Lívia P, Linden, Rafael, Mariante, Rafael M
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Animals, Newborn
Brain - cytology
Calcium-Binding Proteins - metabolism
Cell Adhesion - drug effects
Cell Adhesion - genetics
Cell migration
Cell Movement - drug effects
Cell Movement - genetics
Cells, Cultured
Cytokine
Cytokines - metabolism
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation - drug effects
Gene Expression Regulation - genetics
Inflammation
Lipopolysaccharides - pharmacology
Mice
Mice, Inbred C57BL
Mice, Knockout
Microfilament Proteins - metabolism
Microglia
Microglia - drug effects
Microglia - metabolism
Neurology
Nitric Oxide Synthase Type II - metabolism
Phagocytosis
Phagocytosis - drug effects
Phagocytosis - genetics
Prion protein
Prions - genetics
Prions - metabolism
Protein Transport - drug effects
Protein Transport - genetics
Time Factors
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Summary:Abstract The prion protein (PrPC ) is predominantly expressed in the nervous and immune systems and is involved in relevant cell signaling. Microglia participate in neuroimmune interactions, and their regulatory mechanisms are critical for both health and disease. Despite recent reports with a microglial cell line, little is known about the relevance of PrPC in brain microglia. We investigated the role of PrPC in mouse primary microglia, and found no differences between wild type and Prnp -null cells in cell morphology or the expression of a microglial marker. Translocation of NF-κB to the nucleus also did not differ, nor did cytokine production. The levels of iNOS were also similar and, finally, microglia of either genotype showed no differences in either rates of phagocytosis or migration, even following activation. Thus, functional roles of PrPC in primary microglial cells are — if present — much more subtle than in transformed microglial cell lines.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2015.07.002