BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea

• Published in: Current genetics 2015-11, Vol.61 (4), p.545-553
• Main Authors: Cui, Zhifeng, Gao, Nana, Wang, Qian, Ren, Yun, Wang, Kun, Zhu, Tingheng
• Format: Article
• Language: English
• Subjects: Acetic Acid - metabolism; Acetic Acid - pharmacology; Amino Acid Sequence; Biochemistry; Biomedical and Life Sciences; Botrytis - genetics; Botrytis - metabolism; Botrytis - pathogenicity; Botrytis cinerea; Carbon sources; Cell Biology; Cucumis sativus - drug effects; Cucumis sativus - genetics; Cucumis sativus - metabolism; Cucumis sativus - microbiology; Deoxyribonucleic acid; DNA; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation, Fungal; Lactic Acid - metabolism; Lactic Acid - pharmacology; Life Sciences; Lycopersicon esculentum; Lycopersicon esculentum - drug effects; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - microbiology; Microbial Genetics and Genomics; Microbiology; Molecular Sequence Data; Monocarboxylic Acid Transporters - genetics; Monocarboxylic Acid Transporters - metabolism; Mutagenesis, Insertional; Pathogens; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - microbiology; Plant Sciences; Proteomics; Pyruvic Acid - metabolism; Pyruvic Acid - pharmacology; Research Article; Spores, Fungal - genetics; Spores, Fungal - metabolism; Spores, Fungal - pathogenicity; Tomatoes; Virulence
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s00294-015-0474-1

Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [ B. cinerea MctA ( BcMctA )] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves.