In vitro and in vivo biocompatibility, bioavailability and tolerance of an injectable vehicle for adipose-derived stem/stromal cells for plastic surgery indications

Summary Soft tissue reconstruction is a challenge in plastic surgery, when replacing lost materials and correcting contour defects. Many permanent and temporary fillers have been used to restore the volume of these lesions, but often with poor results and even complications. Adipose-derived stem/str...

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Bibliographic Details
Published in:Journal of plastic, reconstructive & aesthetic surgery reconstructive & aesthetic surgery, 2015-11, Vol.68 (11), p.1491-1497
Main Authors: Lequeux, Charlotte, Rodriguez, Jonathan, Boucher, Fabien, Rouyer, Ondine, Damour, Odile, Mojallal, Ali, Auxenfans, Céline
Format: Article
Language:English
Subjects:
Adipocytes - immunology
Adipocytes - transplantation
Adipose Tissue - cytology
Adipose Tissue - immunology
Adipose tissue engineering
Adipose-derived stem/stromal cells
Animals
Biological Availability
Cell Differentiation
Cell Proliferation
Cells, Cultured
Disease Models, Animal
Humans
Immune Tolerance
Male
Materials Testing
Mice
Mice, Nude
Plastic Surgery
Reconstructive Surgical Procedures - methods
Stem Cell Transplantation - methods
Stromal Cells - immunology
Stromal Cells - transplantation
Tissue Engineering - methods
Vehicle
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Summary:Summary Soft tissue reconstruction is a challenge in plastic surgery, when replacing lost materials and correcting contour defects. Many permanent and temporary fillers have been used to restore the volume of these lesions, but often with poor results and even complications. Adipose-derived stem/stromal cells (ASCs) and adipose tissue engineering have been suggested as valuable alternatives. In order to inject these cultured cells, it was essential to find a suitable vehicle. The purpose of this study was to evaluate Cytocare® , an injectable medical device, composed of hyaluronic acid plus amino acids, vitamins and mineral salts. First, ASC viability and bioavailability in the 3 different available Cytocare® formulations using the MTT test were assessed; then an animal experiment, testing the tolerance after intradermal injections of both Cytocare® alone and with ASCs was carried out. Our in vitro results demonstrate a high biocompatibility of Cytocare® resulting in a better viability of ASCs when cultured in Cytocare® compared to culture medium (p 
ISSN:1748-6815
1878-0539
DOI:10.1016/j.bjps.2015.07.022