Deglycosylation of Toxocara excretory–secretory antigens improves the specificity of the serodiagnosis for human toxocariasis

Summary Serodiagnosis of human toxocariasis is difficult in tropical areas where other helminthiasis are endemic. Many studies have shown that glycans from helminths may be the responsible for cross‐reactions in the immunoassays. In this study, we have evaluated the deglycosylation of the Toxocara c...

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Bibliographic Details
Published in:Parasite immunology 2015-11, Vol.37 (11), p.557-567
Main Authors: Roldán, W.H., Elefant, G. R., Ferreira, A. W.
Format: Article
Language:English
Subjects:
Animals
Antigens, Helminth - blood
Blotting, Western
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay - methods
excretory–secretory antigen
Female
Glycosylation
Helminth Proteins - blood
Humans
immunoglobulin
Immunoglobulin G - blood
Male
Sensitivity and Specificity
serodiagnosis
Toxocara - physiology
toxocariasis
Toxocariasis - diagnosis
Toxocariasis - immunology
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Summary:Summary Serodiagnosis of human toxocariasis is difficult in tropical areas where other helminthiasis are endemic. Many studies have shown that glycans from helminths may be the responsible for cross‐reactions in the immunoassays. In this study, we have evaluated the deglycosylation of the Toxocara canis excretory–secretory (TES) antigens for the detection of IgG antibodies using a panel of 228 serum samples (58 patients with toxocariasis, 75 patients with other helminth infections and 95 healthy individuals) by ELISA and Western blot assays. Our results showed that the deglycosylation of TES antigens resulted in a single fraction of 26 kDa (dTES) and was able to detect IgG antibodies with a sensitivity and specificity of 100% in both above‐mentioned assays. The rate of cross‐reactions, observed in ELISA with TES (13·3%), was significantly reduced (5·3%) when the dTES antigens were used. Likewise, the cross‐reactivity observed with the fractions of 32, 55 and 70 kDa of the TES antigens was totally eliminated when the dTES were used in the Western blot. All these results showed that the deglycosylation of the TES antigens really improves the specificity of the serodiagnosis of human toxocariasis in endemic areas for helminth infections.
ISSN:0141-9838
1365-3024
DOI:10.1111/pim.12248