Relationship between cardiac tissue glycation and skin autofluorescence in patients with coronary artery disease

• Published in: Diabetes & metabolism 2015-11, Vol.41 (5), p.410-415
• Main Authors: Hofmann, B, Jacobs, K, Navarrete Santos, A, Wienke, A, Silber, R.E, Simm, A
• Format: Article
• Language: English
• Subjects: Age Factors; Aged; Aged, 80 and over; AGEs; Animals; Atrial Appendage - metabolism; Biomarkers - metabolism; Collagen - chemistry; Collagen - metabolism; Coronary Artery Bypass; Coronary artery disease; Coronary Artery Disease - blood; Coronary Artery Disease - complications; Coronary Artery Disease - metabolism; Coronary Artery Disease - surgery; Diabetes mellitus; Diabetes Mellitus, Type 2 - complications; Diabetic Angiopathies - blood; Diabetic Angiopathies - metabolism; Diabetic Angiopathies - surgery; Endocrinology & Metabolism; European Continental Ancestry Group; Germany; Glycated Hemoglobin A - analysis; Glycation End Products, Advanced - metabolism; Humans; Internal Medicine; Male; Optical Imaging; Prospective Studies; Skin - metabolism; Skin autofluorescence; Subcutaneous Tissue - metabolism; Up-Regulation
• ISSN: 1262-3636; 1878-1780
• DOI: 10.1016/j.diabet.2014.12.001

Abstract Aim During ageing, advanced glycation end-products (AGEs) accumulate in extracellular matrix proteins like collagen and contribute to a decline in organ function. As skin autofluorescence (sAF) can assess subcutaneous accumulation of fluorescent AGEs, this study aimed to investigate the relationship between AGE-modified cardiac tissue collagen and AGE-related sAF in coronary artery bypass graft (CABG) surgery patients. Methods Between January 2011 and January 2012, data from 72 consecutive male patients undergoing isolated CABG were prospectively recorded. Collagen fractions were isolated from the right atrial appendages of these patients by proteolysis and collagenase digestion. Collagen was quantified by hydroxyproline assay, and AGEs by AGE-related intrinsic fluorescence; sAF was measured using an autofluorescence reader. Results Biochemical analysis showed that the insoluble cardiac collagen fraction contained the highest amounts of accumulated AGEs; the AGE-related intrinsic fluorescence of this fraction increased with age ( P = 0.0001), blood glucose ( P = 0.002), HbA1c ( P = 0.01) and sAF ( P = 0.008). Conclusion This study demonstrated for the first time a relationship between cardiac tissue glycation and AGE-related sAF. In addition, cardiac tissue glycation was associated with age, blood glucose and long-term glucose values in patients with coronary artery disease.