In Vitro and In Vivo Tumor Targeted Photothermal Cancer Therapy Using Functionalized Graphene Nanoparticles

Despite the tremendous progress that photothermal therapy (PTT) has recently achieved, it still has a long way to go to gain the effective targeted photothermal ablation of tumor cells. Driven by this need, we describe a new class of targeted photothermal therapeutic agents for cancer cells with pH...

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Bibliographic Details
Published in:Biomacromolecules 2015-11, Vol.16 (11), p.3519-3529
Main Authors: Kim, Sung Han, Lee, Jung Eun, Sharker, Shazid Md, Jeong, Ji Hoon, In, Insik, Park, Sung Young
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cell Line, Tumor
Dogs
Graphite - chemistry
Graphite - pharmacokinetics
Humans
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacokinetics
Hydrogen-Ion Concentration
Madin Darby Canine Kidney Cells
Methacrylates - chemistry
Methacrylates - pharmacokinetics
Mice
Mice, Inbred BALB C
Mice, Nude
Nanocomposites - chemistry
Nanoparticles - chemistry
Neoplasms - therapy
Phototherapy - methods
Polyethylene Glycols - chemistry
Polyethylene Glycols - pharmacokinetics
Spectroscopy, Near-Infrared
Tissue Distribution
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Summary:Despite the tremendous progress that photothermal therapy (PTT) has recently achieved, it still has a long way to go to gain the effective targeted photothermal ablation of tumor cells. Driven by this need, we describe a new class of targeted photothermal therapeutic agents for cancer cells with pH responsive bioimaging using near-infrared dye (NIR) IR825, conjugated poly­(ethylene glycol)-g-poly­(dimethylaminoethyl methacrylate) (PEG-g-PDMA, PgP), and hyaluronic acid (HA) anchored reduced graphene oxide (rGO) hybrid nanoparticles. The obtained rGO nanoparticles (PgP/HA-rGO) showed pH-dependent fluorescence emission and excellent near-infrared (NIR) irradiation of cancer cells targeted in vitro to provide cytotoxicity. Using intravenously administered PTT agents, the time-dependent in vivo tumor target accumulation was exactly defined, presenting eminent photothermal conversion at 4 and 8 h post-injection, which was demonstrated from the ex vivo biodistribution of tumors. These tumor environment responsive hybrid nanoparticles generated photothermal heat, which caused dominant suppression of tumor growth. The histopathological studies obtained by H&E staining demonstrated complete healing from malignant tumor. In an area of limited successes in cancer therapy, our translation will pave the road to design stimulus environment responsive targeted PTT agents for the safe eradication of devastating cancer.
ISSN:1525-7797
1526-4602
DOI:10.1021/acs.biomac.5b00944