Efficient production of hyperpolarized bicarbonate by chemical reaction on a DNP precursor to measure pH

Purpose To produce hyperpolarized bicarbonate indirectly via chemical reaction from a hyperpolarized precursor and utilize it for the simultaneous regional measurement of metabolism and pH. Methods Alpha keto carboxylic acids are first hyperpolarized by dissolution dynamic nuclear polarization (DNP)...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2015-11, Vol.74 (5), p.1406-1413
Main Authors: Ghosh, Rajat K., Kadlecek, Stephen J., Pourfathi, Mehrdad, Rizi, Rahim R.
Format: Article
Language:English
Subjects:
Animals
Bicarbonates - analysis
Bicarbonates - chemistry
Bicarbonates - metabolism
Carboxylic Acids - analysis
Carboxylic Acids - chemistry
Carboxylic Acids - metabolism
Hydrogen Peroxide - analysis
Hydrogen Peroxide - chemistry
Hydrogen Peroxide - metabolism
Hydrogen-Ion Concentration
hyperpolarized molecules
Lung - chemistry
Magnetic Resonance Spectroscopy - methods
metabolism
Molecular Imaging - methods
pH mapping
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Summary:Purpose To produce hyperpolarized bicarbonate indirectly via chemical reaction from a hyperpolarized precursor and utilize it for the simultaneous regional measurement of metabolism and pH. Methods Alpha keto carboxylic acids are first hyperpolarized by dissolution dynamic nuclear polarization (DNP). These precursor molecules are rapidly reacted with hydrogen peroxide (H2O2) to decarboxylate the species, resulting in new target molecules. Unreacted H2O2 is removed from the system by reaction with sulfite. Interrogation of the ratio of dissolved carbon dioxide (CO2) to bicarbonate can be used to determine pH. Results Conversion of hyperpolarized alpha keto acids to bicarbonate and CO2 results in a minimal loss of the spin order. The reaction can be conducted to completion within seconds and preserves the nuclear spin polarization. Conclusion Through a rapid chemical reaction, we can conserve the nuclear spin order of a DNP precursor to generate multiple hyperpolarized bioprobes otherwise unamenable to polarization. This indirect technique for the production of hyperpolarized agents can be applied to different precursor compounds to generate additional novel probes. Magn Reson Med 74:1406–1413, 2015. © 2014 Wiley Periodicals, Inc.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.25530