Delta-aminolevulinic acid dehydratase activity (ALA-D) in red mullet (Mullus barbatus) from Mediterranean waters as biomarker of lead exposure

The enzyme delta-aminolevulinic acid dehydratase (ALA-D) has been investigated as biomarker of lead (Pb) exposure in red mullet (Mullus barbatus) from the Spanish continental shelf. Concentrations of Pb and Zn in muscle and organosomatic indices were also measured to explore causality. Blood ALA-D a...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety 2015-05, Vol.115, p.209-216
Main Authors: Fernández, B, Martínez-Gómez, C, Benedicto, J
Format: Article
Language:English
Subjects:
Animals
Biomarkers - blood
Biomarkers - metabolism
Environmental Exposure - analysis
Female
Lead - analysis
Male
Marine
Mediterranean Sea
Mullus barbatus
Porphobilinogen Synthase - blood
Porphobilinogen Synthase - metabolism
Seawater
Smegmamorpha - blood
Smegmamorpha - metabolism
Water Pollutants, Chemical - analysis
Zinc - analysis
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Summary:The enzyme delta-aminolevulinic acid dehydratase (ALA-D) has been investigated as biomarker of lead (Pb) exposure in red mullet (Mullus barbatus) from the Spanish continental shelf. Concentrations of Pb and Zn in muscle and organosomatic indices were also measured to explore causality. Blood ALA-D assay conditions were optimized; the optimum pH for this species has been set to 6.5. Results showed that ALA-D activity ranged from 3.2 to 16.9 nmol PBGmin(-1)mg(-1). No significant differences on ALA-D levels between genders have been detected. ALA-D Baseline level and Background Assessment Criteria (BAC) for this species have been set to 9.1 and 6.6 nmol PBGmin(-1)mg(-1), respectively. There have been detected significant differences on ALA-D activity levels among areas, though the markedly low levels of Pb measured in fish muscle seemed not to be able to produce a relevant suppression on ALA-D. In spite of this, a weak inverse relationship detected between ALA-D and Pb concentrations pointed out the potential of this biomarker in red mullet to reflect Pb bioavailability in marine environment. Nevertheless, subsequent research on ALA-D in marine fish species is recommended to be limited to areas where environmental Pb is effectively accumulated by fish.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2015.02.023