Segregation of genotypically diverse progeny from self-fertilized haploids of the Chinese straw mushroom, Volvariella volvacea

The electrophoretic karyotype of the Chinese straw mushroom, Volvariella volvacea, was determined. The haploid strain V34 of V. volvacea has 15 chromosomes ranging in size from 1.4 to 5.1 Mb. No chromosomal polymorphism in terms of size and number was seen in either of two growth stages: vegetative...

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Bibliographic Details
Published in:Mycological research 1999-10, Vol.103 (10), p.1335-1345
Main Authors: CHIU, SIU WAI, MOORE, DAVID
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
basidiospores
Biological and medical sciences
biological development
chromosome polymorphism
Classical and quantitative genetics
Classical and quantitative genetics. Population genetics. Molecular genetics
developmental stages
DNA fingerprinting
fruiting bodies
Fundamental and applied biological sciences. Psychology
fungal anatomy
Generalities. Genetics. Plant material
genetic variation
Genetics and breeding of economic plants
genotype
haploidy
karyotyping
mycelium
protoplasts
regrowth
segregation
self compatibility
Volvariella volvacea
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Summary:The electrophoretic karyotype of the Chinese straw mushroom, Volvariella volvacea, was determined. The haploid strain V34 of V. volvacea has 15 chromosomes ranging in size from 1.4 to 5.1 Mb. No chromosomal polymorphism in terms of size and number was seen in either of two growth stages: vegetative mycelia or fruit-body gill tissues. DNA fingerprints were prepared by the arbitrarily- primed polymerase chain reaction. Those obtained from different stages during fruit-body morphogenesis were identical. However, variation in DNA fingerprints was evident in protoplast regenerants derived from the same vegetative mycelium. Thus the haploid mycelium of strain V34 is heterokaryotic but the bulk genotype is stable during fruit-body development. F1 and F2 progenies germinated from the haploid, uninucleate basidiospores from self-fertilized fruit bodies also regularly segregate a range of mycelial morphological variants as well as phenotypic variation revealed by DNA fingerprints. We overview the known mechanisms to generate genetic variation and propose a novel mechanism that could account for the 1:1 segregation ratio of self-fertile to self-sterile progeny regularly obtained from selfed Volvariella volvacea fruit bodies.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756299001422