Azole resistance in canine and feline isolates of Aspergillus fumigatus

Abstract Azole resistance is an emerging cause of treatment failure in humans with aspergillosis. The aim of this study was to determine if azole resistance is emerging in Aspergillus fumigatus isolates from canine and feline sino-nasal aspergillosis cases. Susceptibilities of isolates collected bet...

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Bibliographic Details
Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2015-10, Vol.42, p.37-41
Main Authors: Talbot, Jessica J, Kidd, Sarah E, Martin, Patricia, Beatty, Julia A, Barrs, Vanessa R
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antifungal Agents - pharmacology
Aspergillosis
Aspergillosis - microbiology
Aspergillosis - veterinary
Aspergillus fumigatus
Aspergillus fumigatus - drug effects
Aspergillus fumigatus - genetics
Aspergillus fumigatus - isolation & purification
Azole resistance
Cat Diseases - microbiology
Cats
Clotrimazole
Cytochrome P-450 Enzyme System - genetics
Dog Diseases - microbiology
Dogs
Drug Resistance, Fungal - genetics
Enilconazole
Fungal Proteins - genetics
Infectious Disease
Itraconazole - pharmacology
Microbial Sensitivity Tests
Mutation
Triazoles - pharmacology
Voriconazole - pharmacology
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Summary:Abstract Azole resistance is an emerging cause of treatment failure in humans with aspergillosis. The aim of this study was to determine if azole resistance is emerging in Aspergillus fumigatus isolates from canine and feline sino-nasal aspergillosis cases. Susceptibilities of isolates collected between 1988 and 2014 from 46 dogs and 4 cats to itraconazole, posaconazole, voriconazole, fluconazole and ketoconazole were assessed using Sensititre YeastOne microdilution trays; and to enilconazole and clotrimazole, following the CLSI M38-A2 standard. For the majority of isolates MICs were high for ketoconazole, low for enilconazole and clotrimazole, and less than established epidemiological cut-off values for itraconazole, posaconazole and voriconazole. One canine isolate from 1992 had multiazole resistance and on C yp51A gene sequencing a mutation associated with azole resistance (F46Y) was detected. There is no evidence of emerging azole resistance among A. fumigatus isolates from dogs and cats and topical azole therapy should be effective against most isolates.
ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2015.08.002