Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria

Cyanobacteria have gained popularity among the metabolic engineering community as a tractable photosynthetic host for renewable chemical production. However, though a number of successfully engineered production systems have been reported, long-term genetic stability remains an issue for cyanobacter...

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Bibliographic Details
Published in:ACS synthetic biology 2015-11, Vol.4 (11), p.1197-1204
Main Authors: Nozzi, Nicole E, Atsumi, Shota
Format: Article
Language:English
Subjects:
Butylene Glycols - analysis
Butylene Glycols - metabolism
Chromatography, Gas
Cyanobacteria - genetics
Genetic Engineering
Genome, Bacterial
Green Fluorescent Proteins - genetics
Plasmids
Synthetic Biology
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Summary:Cyanobacteria have gained popularity among the metabolic engineering community as a tractable photosynthetic host for renewable chemical production. However, though a number of successfully engineered production systems have been reported, long-term genetic stability remains an issue for cyanobacterial systems. The genetic engineering toolbox for cyanobacteria is largely lacking inducible systems for expression control. The characterization of tight regulation systems for use in cyanobacteria may help to alleviate this problem. In this work we explore the function of the IPTG inducible promoter P LlacO1 in the model cyanobacterium Synechococcus elongatus PCC 7942 as well as the effect of gene order within an operon on pathway expression. According to our experiments, P LlacO1 functions well as an inducible promoter in S. elongatus. Additionally, we found that gene order within an operon can strongly influence control of expression of each gene.
ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.5b00057