Molecular identification of schistosome intermediate hosts: case studies of Bulinus forskalii group species (Gastropoda: Planorbidae) from Central and East Africa

• Published in: Biological journal of the Linnean Society 1999-09, Vol.68 (1-2), p.215-240
• Main Authors: JONES, C.S, NOBLE, L.R, OUMA, J, KARIUKI, H.C, MIMPFOUNDI, R, BROWN, D.S, ROLLINSON, D
• Format: Article
• Language: English
• Subjects: biological resistance; Bulinus; Bulinus forskalii; case studies; cytochrome-c oxidase; DNA; epidemiology; Freshwater; gene flow; genes; intermediate hosts; mitochondrial DNA – cytochrome oxidase subunit I – COI – Randomly Amplified Polymorphic DNA – RAPDs – arbitrary primers – phylogenetics – morphology – morphometrics; parasites; Random amplified polymorphic DNA; random amplified polymorphic DNA technique; schistosomiasis; snails
• ISSN: 0024-4066; 1095-8312
• DOI: 10.1006/bijl.1999.0339

African freshwater snails of the genus Bulinus act as intermediate hosts for schistosomes, trematode parasites responsible for medical and veterinary forms of schistosomiasis. The relationship between these snails and their parasites is an intricate one, with particular species of snail susceptible to infection only by certain species of schistosome. In common with other self-fertile hermaphrodite gastropods, Bulinus consists of a number of closely related species complexes with restricted gene flow between populations of each taxon. Consequently, despite their medical and veterinary importance as intermediate hosts, unambiguous identification and differentiation of planorbid snails such as these remains problematic, often confounding attempts to define the distribution and evolutionary relationships of conchologically similar taxa. Here we consider how morphological methods of discrimination can be used in conjunction with molecular based approaches to improve snail identification, thereby achieving a better understanding of the epidemiology of schistosomiasis. Data are presented from Central and East African taxa which illustrate how PCR-based methods have begun to be used in combination with traditional analyses in an integrated approach to characterize the genus Bulinus, specifically the B. forskalii species group. Particular emphasis is given to the analyses of Random Amplified Polymorphic DNA (RAPDs) and the mitochondrial gene cytochrome oxidase I (COI).