Pyrosequencing reveals microbial community profile in anaerobic bio-entrapped membrane reactor for pharmaceutical wastewater treatment

•A novel anaerobic bio-entrapped membrane reactor (AnBEMR) was operated.•AnBEMR was compared to control AnMBR for the pharmaceutical wastewater treatment.•AnBEMR achieved higher COD removal and generated more biogas production.•Similar dominant groups of bacteria and archaea was found in both anaero...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2016-01, Vol.200, p.1076-1079
Main Authors: Ng, Kok Kwang, Shi, Xueqing, Ong, Say Leong, Ng, How Yong
Format: Article
Language:English
Subjects:
Anaerobic membrane bioreactor
Anaerobiosis
Archaea - classification
Archaea - genetics
Bacteria - classification
Bacteria - genetics
Biofuels
Biological Oxygen Demand Analysis
Bioreactors - microbiology
Drug Industry
Entrapped biomass
High salinity
Membranes, Artificial
Microbial analysis
Microbial Consortia - genetics
Microbial Consortia - physiology
Pharmaceutical wastewater
Salinity
Waste Disposal, Fluid - instrumentation
Waste Disposal, Fluid - methods
Waste Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•A novel anaerobic bio-entrapped membrane reactor (AnBEMR) was operated.•AnBEMR was compared to control AnMBR for the pharmaceutical wastewater treatment.•AnBEMR achieved higher COD removal and generated more biogas production.•Similar dominant groups of bacteria and archaea was found in both anaerobic MBR.•Higher abundance of dominant archaeal to degrade organic pollutants found in AnBEMR. In this study, pharmaceutical wastewater with high salinity and total chemical oxygen demand (TCOD) was treated by an anaerobic membrane bioreactor (AnMBR) and an anaerobic bio-entrapped membrane reactor (AnBEMR). The microbial populations and communities were analyzed using the 454 pyrosequencing method. The hydraulic retention time (HRT), membrane flux and mean cell residence time (MCRT) were controlled at 30.6h, 6L/m2h and 100d, respectively. The results showed that the AnBEMR achieved higher TCOD removal efficiency and greater biogas production compared to the AnMBR. Through DNA pyrosequencing analysis, both the anaerobic MBRs showed similar dominant groups of bacteria and archaea. However, phylum Elusimicrobia of bacteria was only detected in the AnBEMR; the higher abundance of dominant archaeal genus Methanimicrococcus found in the AnBEMR could play an important role in degradation of the major organic pollutant (i.e., trimethylamine) present in the pharmaceutical wastewater.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2015.10.100