Calcium entry initiates processes that restore a barrier to dye entry in severed earthworm giant axons

After severance, axons can restore structural barriers that are necessary for recovery of their electrical function. In earthworm myelinated axons, such a barrier to dye entry is mediated by many vesicles and myelin-derived membranous structures. From time-lapse confocal fluorescence and DIC images,...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience letters 1999-09, Vol.272 (3), p.147-150
Main Authors: Blanchette, Adam R, Ballinger, Martis L, Fishman, Harvey M, Bittner, George D
Format: Article
Language:English
Subjects:
Animals
Annelida and closely related phyla: sipuncula. Echiura. Nemertinea
Axons - metabolism
Axons - ultrastructure
Axotomy
Barrier restoration
Biological and medical sciences
Calcium - metabolism
Calcium - physiology
Calcium-initiated processes
Coloring Agents - pharmacokinetics
Dextrans
Fluoresceins
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
Invertebrates
Membrane sealing
Microscopy, Confocal
Microscopy, Fluorescence
Nerve repair
Oligochaeta - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:After severance, axons can restore structural barriers that are necessary for recovery of their electrical function. In earthworm myelinated axons, such a barrier to dye entry is mediated by many vesicles and myelin-derived membranous structures. From time-lapse confocal fluorescence and DIC images, we now report that Ca 2+ entry and not axonal injury per se initiates the processes that form a dye barrier, as well as the subsequent structural changes in this barrier and associated membranous structures. The time required to restore a dye barrier after transection also depends only on the time of Ca 2+ entry.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(99)00544-3