Hydrolytic activity of alpha-amylase in anaerobic digested sludge

Hydrolysis is usually considered to be a rate-limiting step in anaerobic digestion. For improving anaerobic solid waste treatments, it is essential to elucidate the mechanism of hydrolysis. In this study, alpha-amylase, one of the hydrolytic enzymes, was investigated for the elucidation of more prec...

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Bibliographic Details
Published in:Water science and technology 2005-01, Vol.52 (1-2), p.259-266
Main Authors: Higuchi, Y, Ohashi, A, Imachi, H, Harada, H
Format: Article
Language:English
Subjects:
Aeromonas - genetics
Aeromonas - isolation & purification
Aeromonas - metabolism
alpha-Amylases - metabolism
Amylases
Anaerobic digestion
Anaerobic treatment
Bacteria
Bacteria, Anaerobic - genetics
Bacteria, Anaerobic - isolation & purification
Bacteria, Anaerobic - metabolism
Biodegradation
Colony Count, Microbial
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Fluorescence
Fluorescence in situ hybridization
Glucose - metabolism
Hydrolysis
In Situ Hybridization, Fluorescence
Maltose
Maltose - metabolism
Microbiological strains
Microorganisms
Phylogeny
RNA, Bacterial - analysis
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sewage - microbiology
Sludge
Sludge digestion
Solid waste treatment
Solid wastes
Starch
Starch - metabolism
Substrates
Waste Disposal, Fluid
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Summary:Hydrolysis is usually considered to be a rate-limiting step in anaerobic digestion. For improving anaerobic solid waste treatments, it is essential to elucidate the mechanism of hydrolysis. In this study, alpha-amylase, one of the hydrolytic enzymes, was investigated for the elucidation of more precise mechanism of hydrolysis. Alpha-amylase activity of solid starch-degrading bacteria (SDB) was estimated through batch experiments with several different substrates and with distinction between cell-bound and cell-free alpha-amylase. Monitoring of newly isolated strains of SDB was done by fluorescence in situ hybridization. Results indicated that cell-bound alpha-amylase is chiefly responsible for the hydrolysis in the digested sludge, providing very useful information that the contact between microbial cells and solids is significantly important. The activity of alpha-amylase of the digested sludge remained quite low when not required, but increased as they recognized appropriate substrates. Several-fold higher activity was obtained for starch or maltose as compared to glucose only.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2005.0526