Analysis of DNA oxidative damage related to cell proliferation

In vivo and in vitro cell populations exhibit a different sensitivity and a heterogeneous response to many genotoxic agents. Several studies have been carried out to evaluate the possibility that the different sensitivity of the cells is related to their proliferative status. In this study, the sens...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2000-01, Vol.464 (2), p.229-237
Main Authors: Villani, Paola, Altavista, Pier Luigi, Castaldi, Laura, Leter, Giorgio, Cordelli, Eugenia
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell cycle
Chemical mutagenesis
Comet assay
DNA repair
Medical sciences
Oxidative damage
SCGE
Toxicology
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Summary:In vivo and in vitro cell populations exhibit a different sensitivity and a heterogeneous response to many genotoxic agents. Several studies have been carried out to evaluate the possibility that the different sensitivity of the cells is related to their proliferative status. In this study, the sensitivity of proliferating (P) and quiescent (Q) C3H10T1/2 cells to oxidative damage and their repair capability has been investigated by single cell gel electrophoresis (SCGE) and micronucleus test. Furthermore the possibility to simultaneously detect DNA damage and cell cycle position has been evaluated. Our results showed a dose-related increase of DNA damage in exponential and plateau phase cells treated with hydrogen peroxide (doses ranging between 2.5 and 100 μM). DNA damage was almost completely repaired within 2 h after treatment in both culture conditions. The percentage of cells in the various phases of the cell cycle has been determined by comet assay and by flow cytometry, and a good agreement between the results of the two techniques was found. Untreated exponentially growing cells in G1 phase showed a lower tail moment than S and G2/M cells. The same cell cycle dependence was evidenced in cells treated with low doses of H 2O 2, while, at the higher doses, all cells showed a similar level of damage. These results confirm the sensitivity of the Comet Assay in assessing DNA damage, and support its usefulness in evaluating cell cycle-related differential sensitivity to genotoxic agents.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(99)00192-8