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Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging
Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with l -glutathione and l -cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide,...
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| Published in: | Analytical and bioanalytical chemistry 2012-05, Vol.403 (5), p.1343-1352 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Han, Sihai Mu, Ying Zhu, Qiangyuan Gao, Yibo Li, Zuhong Jin, Qinhan Jin, Wei |
| description | Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with
l
-glutathione and
l
-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer.
Figure
In vivo tumor-targeted multispectral fluorescence imaging with Au:CdHgTe quantum dots |
| doi_str_mv | 10.1007/s00216-012-5921-y |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1744719710</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A429886890</galeid><sourcerecordid>A429886890</sourcerecordid><originalsourceid>FETCH-LOGICAL-c543t-cdfafb00fa98a835ecdd2ad5ef687ee4ba1c6faafb190593dc2c50eeff97360b3</originalsourceid><addsrcrecordid>eNqNksFq3DAQhk1paNK0D9BLMfTSi5MZ2ZLl3palbQqBXjZnoZVGxsG2NpId2LePjJNAobSLDhKj758Zaf4s-4RwhQD1dQRgKApAVvCGYXF8k12gQFkwweHt67li59n7GO8BkEsU77JzxqqqTtKLbLeZv23tTbuj_GHW4zQPufVTzJ0PeTfmj92jz1PQh2LSoaWJbD7M_dTFA5kp6D53_ewDRUOjobwbdNuN7YfszOk-0sfn_TK7-_F9t70pbn___LXd3BaGV-VUGOu02wM43UgtS07GWqYtJydkTVTtNRrhdGKwAd6U1jDDgci5pi4F7MvL7Oua9xD8w0xxUkOXOul7PZKfo8I6PRObGuE0FIQo8f8oYyhl2ZQnZAXJEg1wCorASo58Qb-saKt7Ut3ofPpps-BqU7FGSiGbhbr6C5WWpaEzfiTXpfgfAlwFJvgYAzl1CGlg4Zhqq8VPavWTSn5Si5_UMWk-P3c97weyr4oXAyWArUBMV2NLQd37OYxp6v_I-gQUP9SE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1010235150</pqid></control><display><type>article</type><title>Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging</title><source>Springer Link</source><creator>Han, Sihai ; Mu, Ying ; Zhu, Qiangyuan ; Gao, Yibo ; Li, Zuhong ; Jin, Qinhan ; Jin, Wei</creator><creatorcontrib>Han, Sihai ; Mu, Ying ; Zhu, Qiangyuan ; Gao, Yibo ; Li, Zuhong ; Jin, Qinhan ; Jin, Wei</creatorcontrib><description>Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with
l
-glutathione and
l
-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer.
Figure
In vivo tumor-targeted multispectral fluorescence imaging with Au:CdHgTe quantum dots</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-012-5921-y</identifier><identifier>PMID: 22447216</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Animals ; Aspartate ; Biochemistry ; Biocompatibility ; Biomedical materials ; Cadmium Compounds - chemistry ; Cell Line, Tumor ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cysteine ; Female ; Fluorescence ; Food Science ; Humans ; Imaging ; In vivo testing ; In vivo tests ; Laboratory Medicine ; Male ; Markers ; Mercury Compounds - chemistry ; Mice ; Mice, Inbred ICR ; Molecular Imaging - methods ; Monitoring/Environmental Analysis ; Monoclonal antibodies ; Neoplasms - chemistry ; Neoplasms - diagnosis ; Original Paper ; Photoluminescence ; Quantum Dots ; Surgical implants ; Tumors</subject><ispartof>Analytical and bioanalytical chemistry, 2012-05, Vol.403 (5), p.1343-1352</ispartof><rights>Springer-Verlag 2012</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-cdfafb00fa98a835ecdd2ad5ef687ee4ba1c6faafb190593dc2c50eeff97360b3</citedby><cites>FETCH-LOGICAL-c543t-cdfafb00fa98a835ecdd2ad5ef687ee4ba1c6faafb190593dc2c50eeff97360b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22447216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Sihai</creatorcontrib><creatorcontrib>Mu, Ying</creatorcontrib><creatorcontrib>Zhu, Qiangyuan</creatorcontrib><creatorcontrib>Gao, Yibo</creatorcontrib><creatorcontrib>Li, Zuhong</creatorcontrib><creatorcontrib>Jin, Qinhan</creatorcontrib><creatorcontrib>Jin, Wei</creatorcontrib><title>Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with
l
-glutathione and
l
-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer.
Figure
In vivo tumor-targeted multispectral fluorescence imaging with Au:CdHgTe quantum dots</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Aspartate</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Cadmium Compounds - chemistry</subject><subject>Cell Line, Tumor</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cysteine</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Food Science</subject><subject>Humans</subject><subject>Imaging</subject><subject>In vivo testing</subject><subject>In vivo tests</subject><subject>Laboratory Medicine</subject><subject>Male</subject><subject>Markers</subject><subject>Mercury Compounds - chemistry</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Molecular Imaging - methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Monoclonal antibodies</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - diagnosis</subject><subject>Original Paper</subject><subject>Photoluminescence</subject><subject>Quantum Dots</subject><subject>Surgical implants</subject><subject>Tumors</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNksFq3DAQhk1paNK0D9BLMfTSi5MZ2ZLl3palbQqBXjZnoZVGxsG2NpId2LePjJNAobSLDhKj758Zaf4s-4RwhQD1dQRgKApAVvCGYXF8k12gQFkwweHt67li59n7GO8BkEsU77JzxqqqTtKLbLeZv23tTbuj_GHW4zQPufVTzJ0PeTfmj92jz1PQh2LSoaWJbD7M_dTFA5kp6D53_ewDRUOjobwbdNuN7YfszOk-0sfn_TK7-_F9t70pbn___LXd3BaGV-VUGOu02wM43UgtS07GWqYtJydkTVTtNRrhdGKwAd6U1jDDgci5pi4F7MvL7Oua9xD8w0xxUkOXOul7PZKfo8I6PRObGuE0FIQo8f8oYyhl2ZQnZAXJEg1wCorASo58Qb-saKt7Ut3ofPpps-BqU7FGSiGbhbr6C5WWpaEzfiTXpfgfAlwFJvgYAzl1CGlg4Zhqq8VPavWTSn5Si5_UMWk-P3c97weyr4oXAyWArUBMV2NLQd37OYxp6v_I-gQUP9SE</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Han, Sihai</creator><creator>Mu, Ying</creator><creator>Zhu, Qiangyuan</creator><creator>Gao, Yibo</creator><creator>Li, Zuhong</creator><creator>Jin, Qinhan</creator><creator>Jin, Wei</creator><general>Springer-Verlag</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20120501</creationdate><title>Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging</title><author>Han, Sihai ; Mu, Ying ; Zhu, Qiangyuan ; Gao, Yibo ; Li, Zuhong ; Jin, Qinhan ; Jin, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-cdfafb00fa98a835ecdd2ad5ef687ee4ba1c6faafb190593dc2c50eeff97360b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Aspartate</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Cadmium Compounds - chemistry</topic><topic>Cell Line, Tumor</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cysteine</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Food Science</topic><topic>Humans</topic><topic>Imaging</topic><topic>In vivo testing</topic><topic>In vivo tests</topic><topic>Laboratory Medicine</topic><topic>Male</topic><topic>Markers</topic><topic>Mercury Compounds - chemistry</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Molecular Imaging - methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Monoclonal antibodies</topic><topic>Neoplasms - chemistry</topic><topic>Neoplasms - diagnosis</topic><topic>Original Paper</topic><topic>Photoluminescence</topic><topic>Quantum Dots</topic><topic>Surgical implants</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Sihai</creatorcontrib><creatorcontrib>Mu, Ying</creatorcontrib><creatorcontrib>Zhu, Qiangyuan</creatorcontrib><creatorcontrib>Gao, Yibo</creatorcontrib><creatorcontrib>Li, Zuhong</creatorcontrib><creatorcontrib>Jin, Qinhan</creatorcontrib><creatorcontrib>Jin, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Sihai</au><au>Mu, Ying</au><au>Zhu, Qiangyuan</au><au>Gao, Yibo</au><au>Li, Zuhong</au><au>Jin, Qinhan</au><au>Jin, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>403</volume><issue>5</issue><spage>1343</spage><epage>1352</epage><pages>1343-1352</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with
l
-glutathione and
l
-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer.
Figure
In vivo tumor-targeted multispectral fluorescence imaging with Au:CdHgTe quantum dots</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22447216</pmid><doi>10.1007/s00216-012-5921-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Analytical Chemistry Animals Aspartate Biochemistry Biocompatibility Biomedical materials Cadmium Compounds - chemistry Cell Line, Tumor Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cysteine Female Fluorescence Food Science Humans Imaging In vivo testing In vivo tests Laboratory Medicine Male Markers Mercury Compounds - chemistry Mice Mice, Inbred ICR Molecular Imaging - methods Monitoring/Environmental Analysis Monoclonal antibodies Neoplasms - chemistry Neoplasms - diagnosis Original Paper Photoluminescence Quantum Dots Surgical implants Tumors |
| title | Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging |
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