Identification of Ribonucleoprotein (RNP)-Specific Protein Interactions Using a Yeast RNP Interaction Trap Assay (RITA)

We describe an adaptation of the yeast three-hybrid system that allows the reconstitution in vivo of tripartite (protein-RNA-protein) ribonucleoproteins (RNPs). To build and try this system that we called RNP interaction trap assay (RITA), we used as a model the autoantigenic Ro RNPs. hY RNAs bear d...

Full description

Saved in:
Bibliographic Details
Published in:BioTechniques 1999-10, Vol.27 (4), p.790-796
Main Authors: Bouffard, Pascal, Brière, Francis, Wellinger, Raymund J, Boire, Gilles
Format: Article
Language:English
Subjects:
Binding Sites
Biological and medical sciences
Diverse techniques
DNA, Complementary - analysis
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Library
HeLa Cells
Humans
Molecular and cellular biology
Molecular Probe Techniques
Plasmids - genetics
Recombinant Proteins
Ribonucleoproteins
RNA - metabolism
RNP interaction trap assay
Saccharomyces cerevisiae - genetics
Transfection
Two-Hybrid System Techniques
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!