Determination of breath acetone in 149 Type 2 diabetic patients using a ringdown breath-acetone analyzer

• Published in: Analytical and bioanalytical chemistry 2015-02, Vol.407 (6), p.1641-1650
• Main Authors: Sun, Meixiu, Chen, Zhuying, Gong, Zhiyong, Zhao, Xiaomeng, Jiang, Chenyu, Yuan, Yuan, Wang, Zhennang, Li, Yingxin, Wang, Chuji
• Format: Article
• Language: English
• Subjects: Acetone; Acetone - metabolism; Analytical Chemistry; Analyzers; Biochemistry; Blood; Breath tests; Breath Tests - instrumentation; Breathalyzers; Case-Control Studies; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Diabetes; Diabetes Mellitus, Type 2 - metabolism; Diabetics; Elevated; Fittings; Food Science; Gas chromatography; Gas Chromatography-Mass Spectrometry; Glucose; Health aspects; Human subjects; Humans; Identification and classification; Laboratory Medicine; Limit of Detection; Mass spectrometry; Medical equipment; Metabolism; Meters; Monitoring/Environmental Analysis; Patients; Physiological aspects; Research Paper; Scientific imaging; Type 2 diabetes
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-014-8401-8

Over 90 % of diabetic patients have Type 2 diabetes. Although an elevated mean breath acetone concentration has been found to exist in Type 1 diabetes (T1D), information on breath acetone in Type 2 diabetes (T2D) has yet to be obtained. In this study, we first used gas chromatography–mass spectrometry (GC–MS) to validate a ringdown breath-acetone analyzer based on the cavity-ringdown-spectroscopy technique, through comparing breath acetone concentrations in the range 0.5–2.5 ppm measured using both methods. The linear fitting of R  = 0.99 suggests that the acetone concentrations obtained using both methods are consistent with a largest standard deviation of ±0.4 ppm in the lowest concentration of the range. Next, 620 breath samples from 149 T2D patients and 42 healthy subjects were collected and tested using the breath analyzer. Four breath samples were taken from each subject under each of four different conditions: fasting, 2 h post-breakfast, 2 h post-lunch, and 2 h post-dinner. Simultaneous blood glucose levels were also measured using a standard diabetic-management blood-glucose meter. For the 149 T2D subjects, their exhaled breath acetone concentrations ranged from 0.1 to 19.8 ppm; four different ranges of breath acetone concentration, 0.1–19.8, 0.1–7.1, 0.1–6.3, and 0.1–9.5 ppm, were obtained for the subjects under the four different conditions, respectively. For the 42 healthy subjects, their breath acetone concentration ranged from 0.1 to 2.6 ppm; four different ranges of breath acetone concentration, 0.3–2.6, 0.1–2.6, 0.1–1.7, and 0.3–1.6 ppm, were obtained for the four different conditions. The mean breath acetone concentration of the 149 T2D subjects was determined to be 1.5 ± 1.5 ppm, which was 1.5 times that of 1.0 ± 0.6 ppm for the 42 healthy subjects. No correlation was found between the breath acetone concentration and the blood glucose level of the T2D subjects and the healthy volunteers. This study using a relatively large number of subjects provides new data regarding breath acetone in diabetes (T1D and T2D) and suggests that an elevated mean breath acetone concentration also exists in T2D.