AC Electrothermal Circulatory Pumping Chip for Cell Culture

Herein we describe a novel AC electrothermal (ACET) fluidic circulatory pumping chip to overcome the challenge of fluid-to-tissue ratio for “human-on-a-chip” cell culture systems. To avoid the deleterious effects of Joule heating and electric current on sample cells, a rectangular microchannel was d...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2015-12, Vol.7 (48), p.26792-26801
Main Authors: Lang, Qi, Wu, Yanshuang, Ren, Yukun, Tao, Ye, Lei, Lei, Jiang, Hongyuan
Format: Article
Language:English
Subjects:
Cell Culture Techniques - methods
Cell Line, Tumor
Cell Survival
Computer Simulation
Culture Media
Electric Conductivity
Electrochemistry - methods
HEK293 Cells
Humans
Numerical Analysis, Computer-Assisted
Rheology
Temperature
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Summary:Herein we describe a novel AC electrothermal (ACET) fluidic circulatory pumping chip to overcome the challenge of fluid-to-tissue ratio for “human-on-a-chip” cell culture systems. To avoid the deleterious effects of Joule heating and electric current on sample cells, a rectangular microchannel was designed with distantly separated regions for pumping and cell culture. Temperature variations were examined using a commercial thermocouple sensor to detect temperature values in both pumping and culture regions. To generate a sufficient ACET circulatory pumping rate, 30 pairs of asymmetrical electrodes were employed in the pumping region; generated ACET velocity was measured by fluorescent microparticle image velocimetry. The benefits of our pumping chip were demonstrated by culturing human embryonic kidney cells (HEK293T) and human colon carcinoma cells (SW620) for 72 h with an energized voltage of 3 V and 10 MHz. Cells grew and proliferated well, implying our ACET circulatory pumping chip has great potential for cell culture and tissue engineering applications.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.5b08863