Determination of specimen storage conditions for measuring isoagglutinin titers

Abstract Background This study aimed to determine the optimal specimen storage conditions for isoagglutinin titration by changing storage time and temperature. Study design and methods Serum specimens from 60 individuals were stored at room temperature (RT, 25 °C) or at 4 °C and tested every 24 hour...

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Bibliographic Details
Published in:Transfusion and apheresis science 2015-10, Vol.53 (2), p.191-195
Main Authors: Seo, Hee Seoung, Sohn, Ji Yeon, Hwang, Joo-Hyoung, Song, Yoon Kyung, An, Tea-Kyu, Kong, Sun-Young
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Blood Donors
Blood Preservation
Female
Health technology assessment
Hemagglutinins - blood
Hematology, Oncology and Palliative Medicine
Humans
Isoagglutinin
Male
Middle Aged
Refrigeration
Room temperature
Specimen
Storage
Time Factors
Titer
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Summary:Abstract Background This study aimed to determine the optimal specimen storage conditions for isoagglutinin titration by changing storage time and temperature. Study design and methods Serum specimens from 60 individuals were stored at room temperature (RT, 25 °C) or at 4 °C and tested every 24 hours until 96 hours by the immediate spin (IS) method and the anti-human globulin method using dithiothreitol (DTT-AHG). These titer results were compared with the titers which were measured when the samples arrived. The titer endpoint was defined as the highest dilution, with clinically meaningful differences defined as more than 4-fold differences (two serial dilutions) in titer. Results Of the specimens stored at RT for 24, 48, 72, and 96 hours, 5%, 12%, 12%, and 12%, respectively, showed two-fold (one dilution) differences by the IS method, and 8%, 8%, 8%, and 10%, respectively, showed two-fold (one dilution) differences with the DTT-AHG method. Of the specimens refrigerated for 24, 48, 72, and 96 hours, 8%, 10%, 13%, and 13%, respectively, showed two-fold (one dilution differences) by the IS method, and 13%, 12%, 12%, and 12%, respectively, showed two-fold (one dilution) differences with the DTT-AHG method. Conclusions Specimens stored for up to 96 hours at RT and 4 °C showed similar titers using the IS and DTT-AHG methods. These findings suggest that tests can be scheduled regularly.
ISSN:1473-0502
1878-1683
DOI:10.1016/j.transci.2015.03.016