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Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii
Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-conta...
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| Published in: | Plant and cell physiology 2000-01, Vol.41 (1), p.77-83 |
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| Main Authors: | , , , , , , |
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| container_end_page | 83 |
| container_issue | 1 |
| container_start_page | 77 |
| container_title | Plant and cell physiology |
| container_volume | 41 |
| creator | Fujiwara, S. (Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan)) Kobayashi, I Hoshino, S Kaise, T Shimogawara, K Usuda, H Tsuzuki, M |
| description | Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification. |
| doi_str_mv | 10.1093/pcp/41.1.77 |
| format | article |
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(Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan)) ; Kobayashi, I ; Hoshino, S ; Kaise, T ; Shimogawara, K ; Usuda, H ; Tsuzuki, M</creator><creatorcontrib>Fujiwara, S. (Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan)) ; Kobayashi, I ; Hoshino, S ; Kaise, T ; Shimogawara, K ; Usuda, H ; Tsuzuki, M</creatorcontrib><description>Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/41.1.77</identifier><identifier>PMID: 10750711</identifier><language>eng</language><publisher>Japan: Plant and Cell Physiology</publisher><subject>Animals ; arsenate ; Arsenate-resistant ; Arsenate-sensitive ; Arsenates - metabolism ; Arsenates - pharmacology ; ARSENIC COMPOUNDS ; BIOLOGICAL PROPERTIES ; Biomethylation ; Cell Division - drug effects ; Cell Division - genetics ; Cell Extracts - chemistry ; CHLAMYDOMONAS REINHARDTII ; Chlamydomonas reinhardtii - drug effects ; Chlamydomonas reinhardtii - genetics ; Chlamydomonas reinhardtii - isolation & purification ; Chromatography, High Pressure Liquid ; COMPOSE ARSENICAL ; COMPUESTOS ARSENICALES ; Dimethylarsinic acid ; Drug Resistance ; Freshwater ; Mutagenesis, Insertional ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; Phosphates - metabolism ; PROPIEDADES BIOLOGICAS ; PROPRIETE BIOLOGIQUE ; random insertional mutagenesis ; RESISTANCE AUX FACTEURS NUISIBLES ; RESISTANCE TO INJURIOUS FACTORS ; RESISTENCIA A AGENTES DANINOS ; Time Factors</subject><ispartof>Plant and cell physiology, 2000-01, Vol.41 (1), p.77-83</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-d76ad0f43c13d611f2ca07325762c88332e0c69a66b867c14b4cb1bbe9f790813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10750711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujiwara, S. (Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan))</creatorcontrib><creatorcontrib>Kobayashi, I</creatorcontrib><creatorcontrib>Hoshino, S</creatorcontrib><creatorcontrib>Kaise, T</creatorcontrib><creatorcontrib>Shimogawara, K</creatorcontrib><creatorcontrib>Usuda, H</creatorcontrib><creatorcontrib>Tsuzuki, M</creatorcontrib><title>Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.</description><subject>Animals</subject><subject>arsenate</subject><subject>Arsenate-resistant</subject><subject>Arsenate-sensitive</subject><subject>Arsenates - metabolism</subject><subject>Arsenates - pharmacology</subject><subject>ARSENIC COMPOUNDS</subject><subject>BIOLOGICAL PROPERTIES</subject><subject>Biomethylation</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - genetics</subject><subject>Cell Extracts - chemistry</subject><subject>CHLAMYDOMONAS REINHARDTII</subject><subject>Chlamydomonas reinhardtii - drug effects</subject><subject>Chlamydomonas reinhardtii - genetics</subject><subject>Chlamydomonas reinhardtii - isolation & purification</subject><subject>Chromatography, High Pressure Liquid</subject><subject>COMPOSE ARSENICAL</subject><subject>COMPUESTOS ARSENICALES</subject><subject>Dimethylarsinic acid</subject><subject>Drug Resistance</subject><subject>Freshwater</subject><subject>Mutagenesis, Insertional</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>Phosphates - metabolism</subject><subject>PROPIEDADES BIOLOGICAS</subject><subject>PROPRIETE BIOLOGIQUE</subject><subject>random insertional mutagenesis</subject><subject>RESISTANCE AUX FACTEURS NUISIBLES</subject><subject>RESISTANCE TO INJURIOUS FACTORS</subject><subject>RESISTENCIA A AGENTES DANINOS</subject><subject>Time Factors</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpN0E1r3DAQBmBRGprNx6nnlj3lEryZsWzJPpYlnw000ARCLmIsy4la29pI2tL011euQ4kur5AeXphh7CPCCqHmJxu9OSlwhSsp37EFFhKzGkr-ni0AeJ6BrHCX7YXwAyDdOXxguwiyBIm4YI-XwfUUrRuXNLZL_USedDTe_pkfXbckH8xI0WQpgo32l_lHvQk2RBrjcthOESa7fuppeGnd4EYKidgxFbbR2gO201EfzOFr7rO7s9Pb9UV2_e38cv3lOtMlFDFrpaAWuoJr5K1A7HJNIHleSpHrquI8N6BFTUI0lZAai6bQDTaNqTtZQ4V8nx3NvRvvnrcmRDXYoE3f02jcNiicB5cJHs9QexeCN53aeDuQf1EIatqrSntVBSpUctKfX2u3zWDaN3buSyCbQdqJ-f3_n_xPJSSXpbq4f1ClgKv86w1X35P_NPuOnKJHb4O6uslhOoKnQf8CPceNDg</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Fujiwara, S. 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(Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan)) ; Kobayashi, I ; Hoshino, S ; Kaise, T ; Shimogawara, K ; Usuda, H ; Tsuzuki, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-d76ad0f43c13d611f2ca07325762c88332e0c69a66b867c14b4cb1bbe9f790813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>arsenate</topic><topic>Arsenate-resistant</topic><topic>Arsenate-sensitive</topic><topic>Arsenates - metabolism</topic><topic>Arsenates - pharmacology</topic><topic>ARSENIC COMPOUNDS</topic><topic>BIOLOGICAL PROPERTIES</topic><topic>Biomethylation</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - genetics</topic><topic>Cell Extracts - chemistry</topic><topic>CHLAMYDOMONAS REINHARDTII</topic><topic>Chlamydomonas reinhardtii - drug effects</topic><topic>Chlamydomonas reinhardtii - genetics</topic><topic>Chlamydomonas reinhardtii - isolation & purification</topic><topic>Chromatography, High Pressure Liquid</topic><topic>COMPOSE ARSENICAL</topic><topic>COMPUESTOS ARSENICALES</topic><topic>Dimethylarsinic acid</topic><topic>Drug Resistance</topic><topic>Freshwater</topic><topic>Mutagenesis, Insertional</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>Phosphates - metabolism</topic><topic>PROPIEDADES BIOLOGICAS</topic><topic>PROPRIETE BIOLOGIQUE</topic><topic>random insertional mutagenesis</topic><topic>RESISTANCE AUX FACTEURS NUISIBLES</topic><topic>RESISTANCE TO INJURIOUS FACTORS</topic><topic>RESISTENCIA A AGENTES DANINOS</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiwara, S. 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(Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan))</au><au>Kobayashi, I</au><au>Hoshino, S</au><au>Kaise, T</au><au>Shimogawara, K</au><au>Usuda, H</au><au>Tsuzuki, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2000-01</date><risdate>2000</risdate><volume>41</volume><issue>1</issue><spage>77</spage><epage>83</epage><pages>77-83</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.</abstract><cop>Japan</cop><pub>Plant and Cell Physiology</pub><pmid>10750711</pmid><doi>10.1093/pcp/41.1.77</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0781 |
| ispartof | Plant and cell physiology, 2000-01, Vol.41 (1), p.77-83 |
| issn | 0032-0781 1471-9053 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals arsenate Arsenate-resistant Arsenate-sensitive Arsenates - metabolism Arsenates - pharmacology ARSENIC COMPOUNDS BIOLOGICAL PROPERTIES Biomethylation Cell Division - drug effects Cell Division - genetics Cell Extracts - chemistry CHLAMYDOMONAS REINHARDTII Chlamydomonas reinhardtii - drug effects Chlamydomonas reinhardtii - genetics Chlamydomonas reinhardtii - isolation & purification Chromatography, High Pressure Liquid COMPOSE ARSENICAL COMPUESTOS ARSENICALES Dimethylarsinic acid Drug Resistance Freshwater Mutagenesis, Insertional MUTANT MUTANTES MUTANTS Mutation Phosphates - metabolism PROPIEDADES BIOLOGICAS PROPRIETE BIOLOGIQUE random insertional mutagenesis RESISTANCE AUX FACTEURS NUISIBLES RESISTANCE TO INJURIOUS FACTORS RESISTENCIA A AGENTES DANINOS Time Factors |
| title | Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii |
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