Down-regulation of 5S rRNA by miR-150 and miR-383 enhances c-Myc–rpL11 interaction and inhibits proliferation of esophageal squamous carcinoma cells

• Published in: FEBS letters 2015-12, Vol.589 (24), p.3989-3997
• Main Authors: Wang, Xinyu, Ren, Yanli, Wang, Zhiqiong, Xiong, Xiangyu, Han, Sichong, Pan, Wenting, Chen, Hongwei, Zhou, Liqing, Zhou, Changchun, Yuan, Qipeng, Yang, Ming
• Format: Article
• Language: English
• Subjects: 5S rRNA; Animals; c-Myc; Carcinogenesis; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Female; Gene Expression Regulation, Neoplastic; Humans; Mice, Nude; MicroRNAs - metabolism; miR-150; miR-383; Neoplasm Proteins - agonists; Neoplasm Proteins - metabolism; Neoplasm Transplantation; Proto-Oncogene Proteins c-myc - agonists; Proto-Oncogene Proteins c-myc - metabolism; Ribosomal Proteins - agonists; Ribosomal Proteins - metabolism; RNA - metabolism; RNA Interference; RNA, Neoplasm - antagonists & inhibitors; RNA, Neoplasm - metabolism; RNA, Ribosomal, 5S - antagonists & inhibitors; RNA, Ribosomal, 5S - metabolism; Tumor Burden
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2015.11.012

•We identified a novel layer of 5S rRNA regulation.•miR-150 and miR-383 inhibit esophageal cancer proliferation by silencing of 5S rRNA.•5S rRNA silencing by miR-150 and miR-383 might intensify rpL11–c-Myc interaction. 5S rRNA plays an important part in ribosome biology and is over-expression in multiple cancers. In this study, we found that 5S rRNA is a direct target of miR-150 and miR-383 in esophageal squamous cell carcinoma (ESCC). Overexpression of miR-150 and miR-383 inhibited ESCC cell proliferation in vitro and in vivo. Moreover, 5S rRNA silencing by miR-150 and miR-383 might intensify rpL11–c-Myc interaction, which attenuated role of c-Myc as an oncogenic transcriptional factor and dysregulation of multiple c-Myc target genes. Taken together, our results highlight the involvement of miRNAs in ribosomal regulation during tumorigenesis.