Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated Douglas-Firby novel and commercial fungal cellulases

Softwood residues are the most abundant feedstock available for bioconversion in many northern countries. However, the high costs for delignification and enzymatic hydrolysis currently deter commercialization of softwood bioconversion processes. This study evaluates the abilities of two novel fungal...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2005, Vol.121-124 (1-3), p.219-230
Main Authors: Kurabi, Arwa, Berlin, Alex, Gilkes, Neil, Kilburn, Douglas, Bura, Renata, Robinson, Jamie, Markov, Aleksandr, Skomarovsky, Anton, Gusakov, Aleksandr, Okunev, Oleg, Sinitsyn, Arkady, Gregg, David, Xie, Dan, Saddler, John
Format: Article
Language:English
Subjects:
Biodegradation, Environmental
Cellulases - chemistry
Enzyme Activation
Ethanol - chemistry
Hydrolysis
Lignin - chemistry
Penicillium - enzymology
Pseudotsuga - chemistry
Trichoderma - enzymology
Water - chemistry
Wood
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Summary:Softwood residues are the most abundant feedstock available for bioconversion in many northern countries. However, the high costs for delignification and enzymatic hydrolysis currently deter commercialization of softwood bioconversion processes. This study evaluates the abilities of two novel fungal preparations (MSUBC1 and MSUBC2) and two commercial cellulase preparations (TR1 and TR2) to hydrolyze cellulose in Douglas-firpretreated by steam explosion or ethanol organosolv process. MSUBC1 showed significantly better performance than the other preparations on both lignocellulosic substrates. In particular, MSUBC1 achieved >76% cellulose conversion for hydrolysis of steam-exploded Douglas-fir (approximately 44% lignin) after 72 h at low enzyme loading (10 filter paper units/g of cellulose) and without beta-glucosidase supplementation.
ISSN:0273-2289
0273-2289
DOI:10.1385/ABAB:121:1-3:0219